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Treatments were given orally (po) once daily for 10 days. Liver injury was induced by administration of ethanol (30%, po) for 10 days, once daily, followed by a single administration of LPS (2\u2009mg\/kg, ip) 24\u2009h before euthanasia. After the treatment period, animals were euthanized, and liver and blood samples were collected.\u00a0NAC, but not SIL, prevented the increase in oxalacetic glutamic transaminase (OGT) and pyruvic glutamic transaminase (PGT) serum levels. NAC and SIL did not restore levels of reduced glutathione or hepatic malonaldehyde. The treatments with NAC or SIL showed no difference in the activity of glutathione S\u2010transferase, superoxide dismutase, and catalase compared to vehicle group. Myeloperoxidase and N\u2010acetylglucosaminidase activities are increased, as well as the IL\u20106 and IL\u201010 levels in the liver. The treatment with NAC, but not SIL, reduced the N\u2010acetylglucosamines\u00a0activity and the IL\u20106 and IL\u201010 amount in the liver. Histological findings revealed microsteatosis in the vehicle group, which was not prevented by SIL but was partially reduced in animals receiving NAC.\u00a0Unlike other liver injury models, NAC (200\u2009mg\/kg) or SIL (200\u2009mg\/kg) did not positively affect antioxidant patterns in liver tissue of animals exposed to ethanol plus LPS, but NAC treatment displays anti\u2010inflammatory properties in this model.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jbt.23560","type":"journal-article","created":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T05:52:56Z","timestamp":1697781176000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Are silymarin and N\u2010acetylcysteine able to prevent liver damage mediated by multiple factors? 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