{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T23:34:39Z","timestamp":1787787279476,"version":"build-2784847793"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T00:00:00Z","timestamp":1698364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nonalcoholic fatty liver disease (NAFLD) is an abnormal lipid accumulation disease in hepatocytes. The existing drugs for NAFLD have some side effects, so new therapeutic agents are required to be explored. In this study, the effect and mechanism of icariin (ICA) on high\u2010fat diet\u2010induced NAFLD were investigated. Firstly, a high\u2010fat diet was used to construct a NAFLD rat model and HepG2 cells were treated with 1\u2009mM free fatty acid (FFA). After ICA treatment, the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBil), triglyceride (TG), total cholesterol (TC), low\u2010density lipoprotein cholesterol (LDL\u2010C) and high\u2010density lipoprotein cholesterol (HDL\u2010C) were measured; liver injury and lipid deposition were observed by H&amp;E and Oil Red O staining; interleukin\u20101\u03b2 (IL\u20101\u03b2), IL\u201012, and IL\u20106 were measured by enzyme\u2010linked immunosorbent assay. Additionally, qRT\u2010PCR and western blot were performed to detect miR\u2010206 expression and NF\u2010\u03baB\/MAPK pathway\u2010related protein expression in liver tissues and cells. After a variety of trials, we discovered that compared with the NAFLD group, ICA significantly reduced ALT, AST, TBil, TG, TC, and LDL\u2010C levels and increased HDL\u2010C levels, and improved liver tissue injury and lipid deposition. Moreover, ICA reduced IL\u20101\u03b2, IL\u201012, and IL\u20106 levels in liver tissues and cells as well as inhibited MAPK and NF\u2010\u03baB\u2010related protein expression in the liver tissues. Notably, ICA could significantly increase miR\u2010206 expression in liver tissues and cells. Further experiments confirmed that inhibition of miR\u2010206 was able to reverse the effect of ICA on NAFLD. In conclusion, ICA can alleviate NAFLD by upregulating miR\u2010206 to mediate NF\u2010\u03baB and MAPK pathways.<\/jats:p>","DOI":"10.1002\/jbt.23566","type":"journal-article","created":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T08:36:14Z","timestamp":1698395774000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Icariin alleviates high\u2010fat diet\u2010induced nonalcoholic fatty liver disease via up\u2010regulating miR\u2010206 to mediate NF\u2010\u03baB and MAPK pathways"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0523-0721","authenticated-orcid":false,"given":"Hang","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of General Surgery Gastric Ward II The First Affiliated Hospital of Jinzhou Medical University Jinzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3945-8038","authenticated-orcid":false,"given":"Hongyang","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Jinzhou Medical University Jinzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,27]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"1230","volume":"72","author":"Jing X.","year":"2020","journal-title":"Diabetes Metab. 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