{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:44:24Z","timestamp":1761515064319,"version":"3.43.0"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T00:00:00Z","timestamp":1701043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Urinary tract infection (UTI) mainly results from bacterial infections in the urinary tract and markedly impacts the normal lives of millions of patients worldwide. The infection and damage to urethral epithelial cells is the first and key step of UTI development and is a critical target for treating clinical UTI. Oxybutynin, an agent for treating urinary incontinence, is recently claimed with protective effects on bladder ultrastructure. Our study will assess the impact of Oxybutynin on inflammation in lipopolysaccharide (LPS)\u2010stimulated bladder epithelial cells. Bladder epithelial T24 cells were treated with 1\u2009\u03bcg\/mL LPS with or without 10 and 20\u2009\u03bcM Oxybutynin for 24\u2009h. Increased levels of oxidative stress (OS) biomarkers, such as reactive oxygen species, 8\u2010hydroxy\u20102'\u2010deoxyguanosine, malondialdehyde, as well as upregulated inducible nitric oxide synthase and promoted release of nitric oxide, were observed in LPS\u2010managed T24 cells, all of which were signally suppressed by Oxybutynin. Furthermore, severe inflammatory responses, including enhanced release of cytokines, upregulated matrix metallopeptidase\u20102 (MMP\u20102) and MMP\u20109, and raised monocyte chemoattractant protein\u20101 level, were found in LPS\u2010challenged T24 cells, which were markedly reversed by Oxybutynin. Moreover, the activated toll\u20101ike receptor 4\/nuclear factor\u2010\u03baB pathway observed in LPS\u2010managed T24 cells was repressed by Oxybutynin. Collectively, Oxybutynin mitigated LPS\u2010induced inflammatory response in human bladder epithelial cells.<\/jats:p>","DOI":"10.1002\/jbt.23584","type":"journal-article","created":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T18:00:30Z","timestamp":1701108030000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Oxybutynin ameliorates LPS\u2010induced inflammatory response in human bladder epithelial cells"],"prefix":"10.1002","volume":"38","author":[{"given":"Cheng","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Pharmacy The First People's Hospital of Yibin Yibin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiangtao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology The First People's Hospital of Yibin Yibin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Engineering, School of Pharmacy and Bioengineering Chongqing University of Technology Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy The First People's Hospital of Yibin Yibin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy The First People's Hospital of Yibin Yibin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7458-2494","authenticated-orcid":false,"given":"Zhihua","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Engineering, School of Pharmacy and Bioengineering Chongqing University of Technology Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,11,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mop.0000193276.39495.0d"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3432"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.4081\/aiua.2017.2.134"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2010.101"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/17460441.2017.1331216"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02205.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.6.3179-3186.2004"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.2.1148"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-012-1915-8"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/dth.14344"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.24373"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.23066"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-008-0745-1"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.906835"},{"issue":"4","key":"e_1_2_9_16_1","first-page":"1039","volume":"36","author":"Cheng Y. 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