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Pesticides are one of etiologies that support NAFLD establishment. Therefore, the effects of the insecticide fipronil on the lipid metabolism of the human hepatic cell line, HepG2, was investigated, considering its widespread use in field crops and even to control domestic pests. To address the goals of the study, biochemical, cellular, and molecular analyses of different concentrations of fipronil in cell cultures were investigated, after 24\u2009h of incubation. Relevant metabolites such as triglycerides, glucose levels, \u03b2\u2010oxidation processes, and gene expression of relevant elements correlated with lipid and metabolism of xenobiotics were investigated. The results suggested that at 20\u2009\u03bcM, the pesticide increased the accumulation of triglycerides and neutral lipids by reducing fatty acid oxidation and increasing de novo lipogenesis. In addition, changes were observed in genes that control oxidative stress and the xenobiotic metabolism. Together, the results suggest that the metabolic changes caused by the insecticide fipronil may be deleterious if persistent, favoring the establishment of hepatic steatosis.<\/jats:p>","DOI":"10.1002\/jbt.23595","type":"journal-article","created":{"date-parts":[[2023,12,5]],"date-time":"2023-12-05T02:00:50Z","timestamp":1701741650000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Cellular and molecular effects of fipronil in lipid metabolism of HepG2 and its possible connection to non\u2010alcoholic fatty liver disease"],"prefix":"10.1002","volume":"38","author":[{"given":"Camila C.","family":"Pansa","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio Sinaliza\u00e7\u00e3o Celular e Express\u00e3o G\u00eanica, DBGA, Instituto de Bioci\u00eancias Universidade Estadual Paulista, UNESP  Rio Claro SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Let\u00edcia R.","family":"Molica","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Sinaliza\u00e7\u00e3o Celular e Express\u00e3o G\u00eanica, DBGA, Instituto de Bioci\u00eancias Universidade Estadual Paulista, UNESP  Rio Claro SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiano C.","family":"de Oliveira J\u00fanior","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Sinaliza\u00e7\u00e3o Celular e Express\u00e3o G\u00eanica, DBGA, Instituto de Bioci\u00eancias Universidade Estadual Paulista, UNESP  Rio Claro SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara C.","family":"Santello","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Ambiental, DBGA, Instituto de Bioci\u00eancias Universidade Estadual Paulista, UNESP  Rio Claro SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6838-8393","authenticated-orcid":false,"given":"Karen C. 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