{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T17:35:43Z","timestamp":1789148143448,"version":"build-2803163510"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,26]],"date-time":"2023-12-26T00:00:00Z","timestamp":1703548800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gestational diabetes mellitus (GDM),\u00a0a prevalent complication during the gestation period, has been linked to impaired proliferation and migration of trophoblasts causing placental maldevelopment. We previously found that lncRNA X\u2010inactive specific transcript (XIST)\u00a0played an essential role in GDM progression. Here, we investigated the precise biological functions as well as the upstream and downstream regulatory mechanisms of XIST in GDM. We found that XIST\u00a0and forkhead\u00a0box O1 (FOXO1) were conspicuously upregulated and miR\u2010497\u20105p and methyltransferase\u2010like 14 (METTL14) were downregulated in the placentas of GDM patients. XIST silencing facilitated proliferation and migration and inhibited cell apoptosis and cell cycle arrest in HG\u2010cultured HTR8\/SVneo cells. METTL14 inhibited XIST expression through m6A methylation modification. XIST overexpression abrogated the positive effect of METTL14 overexpression on HG\u2010cultured HTR8\/SVneo cell progression. MiR\u2010497\u20105p and FOXO1 are downstream regulatory genes of XIST in HTR8\/SVneo cells. Reverse experiments illustrated that XIST mediated HTR8\/SVneo cell functions by regulating the miR\u2010497\u20105p\/FOXO1 axis. Additionally, XIST silencing augmented glucose tolerance and alleviated fetal detrimental changes in GDM rats. To conclude, METTL14\u2010mediated XIST silencing facilitated proliferation and migration and inhibited cell apoptosis and cell cycle arrest in HG\u2010cultured HTR8\/SVneo cells via the miR\u2010497\u20105p\/FOXO1 axis, thereby alleviating GDM progression in rats.<\/jats:p>","DOI":"10.1002\/jbt.23621","type":"journal-article","created":{"date-parts":[[2023,12,26]],"date-time":"2023-12-26T07:09:09Z","timestamp":1703574549000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["METTL14\u2010mediated lncRNA XIST silencing alleviates GDM progression by facilitating trophoblast cell proliferation and migration via the miR\u2010497\u20105p\/FOXO1 axis"],"prefix":"10.1002","volume":"38","author":[{"given":"Yanchuan","family":"Li","sequence":"first","affiliation":[{"name":"Obstetrical Department Shaanxi Provincial People's Hospital  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfeng","family":"Liu","sequence":"additional","affiliation":[{"name":"General Surgery, The Second Affiliated Hospital Xi'an Medical University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Yao","sequence":"additional","affiliation":[{"name":"Medical Services Shaanxi Provincial People's Hospital  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haili","family":"Wang","sequence":"additional","affiliation":[{"name":"Obstetrical Department Shaanxi Provincial People's Hospital  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyun","family":"Shi","sequence":"additional","affiliation":[{"name":"Obstetrical Department Shaanxi Provincial People's Hospital  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2961-0913","authenticated-orcid":false,"given":"Meiqing","family":"He","sequence":"additional","affiliation":[{"name":"Ultrasound Department Shaanxi Provincial People's Hospital  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,26]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-8587(20)30161-3"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222312722"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/18756212MTA3qNDApy"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566524019666190520100422"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/21655979.2021.2001205"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.2147\/DMSO.S325993"},{"key":"e_1_2_11_8_1","volume":"2022","author":"Li W.","year":"2022","journal-title":"Comput. 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