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In this work, we investigated the action of carnosine (CAR), a natural dipeptide with several protection roles against oxidative stress in the liver of NAFLD rats. NAFLD was induced by\u00a0WD\u2010rich\u00a0sugars and fat, verifying the histological evidence of steatosis. As intraperitoneal administration of CAR reversed liver steatosis, the protein profiles of NAFLD liver and CAR NAFLD liver were evaluated by label\u2010free proteomics approach. A total of 2531 proteins were identified and the 230 and 276 were significantly up\u2010 and downregulated, respectively, by CAR treatment of NAFLD rats and involved in fundamental pathways such as oxidative stress and lipid metabolism. Perilipin 2 and apolipoprotein E, components of the plasma membrane of vesicle, resulted in highly downregulated in the CAR\u2010treated NAFLD liver. The advanced bioanalytical approach demonstrated the efficacy of CAR in overcoming the main symptoms of NAFLD, ameliorating the steatosis in the liver.<\/jats:p>","DOI":"10.1002\/jbt.23644","type":"journal-article","created":{"date-parts":[[2024,1,27]],"date-time":"2024-01-27T14:53:50Z","timestamp":1706367230000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Quantitative proteomics study of carnosine effect in an animal model of Western diet\u2010induced nonalcoholic fatty liver disease"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4028-0014","authenticated-orcid":false,"given":"Fernando","family":"Moreto","sequence":"first","affiliation":[{"name":"Medical School Sao Paulo State University Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3670-243X","authenticated-orcid":false,"given":"J\u00e9ssica Leite","family":"Garcia","sequence":"additional","affiliation":[{"name":"Medical School Sao Paulo State University Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana L\u00facia dos Anjos","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Medical School Sao Paulo State University Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Radrezza","sequence":"additional","affiliation":[{"name":"Molecular Cell Biology and Genetics Division Max Planck Institute Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariane R\u00f3vero","family":"Costa","sequence":"additional","affiliation":[{"name":"Medical School Sao Paulo State University Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guilherme Ribeiro","family":"Romualdo","sequence":"additional","affiliation":[{"name":"Medical School Sao Paulo State University Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nubia Alves","family":"Grandini","sequence":"additional","affiliation":[{"name":"Medical School Sao Paulo State University Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giancarlo","family":"Aldini","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila Renata","family":"Correa","sequence":"additional","affiliation":[{"name":"Medical School Sao Paulo State University Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8147-7235","authenticated-orcid":false,"given":"Alfonsina","family":"D'Amato","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,1,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/03602532.2017.1293683"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-019-03011-w"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-022-01410-5"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox8080276"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.22751"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23169025"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/10715762.2020.1856830"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2021.07.010"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23031468"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.111.038562"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R000034"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M004515"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.759795"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2006.05357.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M700359-JLR200"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27144452"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40495-017-0081-6"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2002.06041.x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu9040387"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc14-1239"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2015-1966"},{"issue":"4","key":"e_1_2_9_23_1","first-page":"313","volume":"77","author":"Boldyrev A. 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