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Boldine, an alkaloid isolated from <jats:italic>Peumus boldus<\/jats:italic>, has been shown to have antioxidant and anti\u2010inflammatory effects. Currently, there is no definitive treatment option available for CLD. Therefore, we investigated the hepatoprotective effect of boldine against carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>)\u2010induced chronic liver injury in rats. CCl<jats:sub>4<\/jats:sub> (2\u2009mL\/kg., b.w., i.p.) was administered twice weekly for 5 weeks to induce chronic liver injury in rats. Separate groups of rats were given boldine (20\u2009mg\/kg b.w., and 40\u2009mg\/kg b.w.) and silymarin (100\u2009mg\/kg b.w.) orally, daily. Serum transaminases, lipid peroxidation, and antioxidant levels were measured, and nuclear factor\u2010\u03baB (NF\u2010\u03baB), tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1), cyclooxygenase\u20102 (cox\u20102), interleukin\u20101 \u03b2 (IL\u20101\u03b2), and \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA) gene and protein expressions were evaluated. CCl<jats:sub>4<\/jats:sub> administration increased liver marker enzymes of hepatotoxicity in serum and oxidative stress markers, inflammatory genes and \u03b1\u2010smooth muscle actin expression in liver tissue. Boldine concurrent treatment suppressed CCl<jats:sub>4<\/jats:sub>\u2010induced elevation of transaminase levels in serum, restored enzymic and non\u2010enzymic antioxidants, and downregulated NF\u2010\u03baB, TNF\u2010\u03b1, Cox\u20102 and IL\u20101\u03b2 expressions, thereby suppressing hepatic inflammation. Boldine administration also repressed \u03b1\u2010SMA expression. The results of this study demonstrate the antioxidant, anti\u2010inflammatory, and antifibrotic properties of boldine, and it can be a potential therapeutic candidate in the treatment of CLD.<\/jats:p>","DOI":"10.1002\/jbt.23691","type":"journal-article","created":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T07:40:02Z","timestamp":1710834002000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Boldine protects against carbon tetrachloride\u2010induced chronic liver injury by regulating NF\u2010\u03baB signaling pathway"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5068-2383","authenticated-orcid":false,"given":"Devaraj","family":"Ezhilarasan","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Hepatology and Molecular Medicine Lab, Saveetha Dental College Saveetha Institute of Medical and Technical Sciences  Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9313-884X","authenticated-orcid":false,"given":"Karthik","family":"Shree Harini","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Hepatology and Molecular Medicine Lab, Saveetha Dental College Saveetha Institute of Medical and Technical Sciences  Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5845-4932","authenticated-orcid":false,"given":"Munusamy","family":"Karthick","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Hepatology and Molecular Medicine Lab, Saveetha Dental College Saveetha Institute of Medical and Technical Sciences  Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9334-400X","authenticated-orcid":false,"given":"Prathap","family":"Lavanya","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Saveetha Dental College Saveetha Institute of Medical and Technical Sciences  Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.09.019"},{"issue":"3","key":"e_1_2_10_3_1","first-page":"205","volume":"14","author":"Knell A. 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