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We used quantitative reverse transcription\u2010polymerase chain reaction assays to measure miR\u2010206 expression levels in peripheral blood samples from patients with prolactinoma before and after curcumin treatment. We also investigated the proliferation level, viability, and invasion ability of groups of cells treated with different concentrations of curcumin using 3\u2010(4,5)\u2010dimethylthiahiazo (\u2010z\u2010y1)\u20103\u2010di\u2010phenytetrazoliumromide (MTT) assays, cell cloning assays, and Transwell assays, respectively. Furthermore, we determined the levels of autophagy\u2010related proteins and protein kinase B\/mammalian target of the rapamycin (Akt\/mTOR) signaling pathway\u2010related proteins in each group of treated cells by western blot. Curcumin treatment upregulated miR\u2010206 expression levels in the peripheral blood of patients with prolactinoma and in GH3 cells. Knockdown of miR\u2010206 expression enhanced the proliferation and invasive ability of GH3 cells, while curcumin treatment effectively inhibited the aberrant biological behavior of GH3 cells enhanced by miR\u2010206 knockdown. miR\u2010206 knockdown also activated the Akt\/mTOR signaling pathway and inhibited autophagy in GH3 cells, and these changes were effectively reversed by curcumin treatment. Thus, curcumin inhibited the Akt\/mTOR signaling pathway and promoted cell autophagy by miR\u2010206 upregulation, resulting in antitumor effects that inhibited prolactinoma cell proliferation and invasion.<\/jats:p>","DOI":"10.1002\/jbt.23734","type":"journal-article","created":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T04:04:57Z","timestamp":1716177897000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Curcumin affects autophagy of prolactinoma cells by upregulating miR\u2010206 to exert antitumor effects"],"prefix":"10.1002","volume":"38","author":[{"given":"Jia\u2010feng","family":"Duan","sequence":"first","affiliation":[{"name":"Department of Neurology Baoshan Hospital Affiliated to Shanghai University of Traditional Chinese Medicine Shanghai 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