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The functions of Tilianin and sufentanil on NSCLC cell viability, apoptosis, mitochondrial dysfunction, and immunity in vitro were examined using Cell Counting Kit\u20108 assay, flow cytometry, reactive oxygen species level analysis, CD8+ T cell percentage analysis, Western blot, and enzyme\u2010linked immunosorbent assay, respectively. The molecular mechanism regulated by Tilianin and sufentanil in NSCLC was assessed using Western blot, and immunofluorescence assays. Meanwhile, the roles of Tilianin and sufentanil in NSCLC tumor growth, apoptosis, and immunity in vivo were determined by establishing a tumor xenograft mouse model, immunohistochemistry, and Western blot assays. When sufentanil concentration was proximity 2\u2009nM, the inhibition rate of NSCLC cell viability was 50%. The IC50 for A549 cells was 2.36\u2009nM, and the IC50 for H1299 cells was 2.18\u2009nM. The IC50 of Tilianin for A549 cells was 38.7\u2009\u03bcM, and the IC50 of Tilianin for H1299 cells was 44.6\u2009\u03bcM. Functionally, 0.5 nM sufentanil and 10 \u03bcM Tilianin reduced NSCLC cell (A549 and H1299) viability in a dose\u2010dependent manner. Also, 0.5\u2009nM sufentanil and 10\u2009\u03bcM Tilianin enhanced NSCLC cell apoptosis, yet this impact was strengthened after a combination of Tilianin and Sufentanil. Furthermore, 0.5\u2009nM sufentanil and 10\u2009\u03bcM Tilianin repressed NSCLC cell mitochondrial dysfunction and immunity, and these impacts were enhanced after a combination of Tilianin and Sufentanil. Mechanistically, 0.5\u2009nM sufentanil and 10\u2009\u03bcM Tilianin repressed the NF\u2010\u03baB pathway in NSCLC cells, while this repression was strengthened after a combination of Tilianin and Sufentanil. <jats:italic>In vivo<\/jats:italic> experimental data further clarified that 1\u2009\u00b5g\/kg sufentanil and 10\u2009mg\/kg Tilianin reduced NSCLC growth, immunity, and NF\u2010\u03baB pathway\u2010related protein levels, yet these trends were enhanced after a combination of Tilianin and Sufentanil. Tilianin strengthened the antitumor effect of sufentanil in NSCLC.<\/jats:p>","DOI":"10.1002\/jbt.23761","type":"journal-article","created":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T05:35:55Z","timestamp":1719898555000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Tilianin enhances the antitumor effect of sufentanil on non\u2010small cell lung cancer"],"prefix":"10.1002","volume":"38","author":[{"given":"Huixia","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Handan Central Hospital Handan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulin","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Anesthesia Operation Center The Second Affiliated Hospital of Inner Mongolia Medical University Hohhot China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiazheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Handan Central Hospital Handan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yike","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Handan Central Hospital Handan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongxue","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Handan Central Hospital Handan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinbo","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Handan Central Hospital Handan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hangyu","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Handan Central Hospital Handan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0430-7081","authenticated-orcid":false,"given":"Xinyan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Handan Central Hospital Handan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ro.2019.10.003"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/CM9.0000000000002529"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1916623"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature25183"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2020.1226"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000011552"},{"key":"e_1_2_10_8_1","first-page":"1","volume":"2022","author":"Huang H.","year":"2022","journal-title":"Int. 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