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This study investigated the protective effect of curcumin (CURC) and berberine (BER) on CYP\u2010induced cardiac damage. Thirty\u2010six male rats were equally divided into the control, dimethyl sulfoxide (DMSO), CYP, CYP\u2009+\u2009CURC, CYP\u2009+\u2009BER and CYP\u2009+\u2009BER\u2009+\u2009CURC groups. Troponin\u2010I, Creatine kinase\u2010myocardial band (CK\u2010MB), total cholesterol, triglyceride levels in serum samples, and reactive oxygen species (ROS), poly(ADP\u2010ribose) polymerase\u20101 (PARP\u20101), and transient receptor potential melastatin 2 (TRPM2) channel levels in heart tissue were measured using an enzyme\u2010linked immunoassay (ELISA) kit. In addition, histopathological examination and immunohistochemical investigation of the TRPM2 channel, fibroblast specific protein\u20101 (FSP1), transforming growth factor\u2010beta\u2010 1 (TGF\u2010\u03b21) and \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA) expressions were determined in heart tissue. The CYP group's troponin\u2010I, total cholesterol, triglyceride, CK\u2010MB, ROS, PARP\u20101 and TRPM2 channel levels were higher than in the other groups in the ELISA measurements (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). In contrast, these parameters in the group treated with CURC and BER together with CYP were lower than in the CYP group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Additionally, CUR and BER reduced CYP\u2010induced pathological damage, TRPM2, FSP1, TGF\u2010\u03b21 and \u03b1\u2010SMA expressions. The data showed that CYP administration can cause cardiac damage by increasing the TRPM2 channel, TGF\u2010\u03b21, FSP1 and \u03b1\u2010SMA expression levels. Therefore, we concluded that CURC and BER administration following CYP application may be used as therapeutic agents to prevent CYP\u2010induced cardiac damage.<\/jats:p>","DOI":"10.1002\/jbt.23783","type":"journal-article","created":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T08:59:49Z","timestamp":1721984389000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The effects of berberine and curcumin on cardiac, lipid profile and fibrosis markers in cyclophosphamide\u2010induced cardiac damage: The role of the TRPM2 channel"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7623-1492","authenticated-orcid":false,"given":"Z\u00fcbeyir","family":"Huyut","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine Van Yuzuncu Yil University  Van Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6585-4010","authenticated-orcid":false,"given":"Kenan","family":"Yildizhan","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Faculty of Medicine Van Yuzuncu Yil University  Van Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fikret","family":"Alt\u0131nda\u011f","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Van Yuzuncu Yil University  Van Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omsc.2020.100182"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.839464"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11020390"},{"key":"e_1_2_10_5_1","volume-title":"Current Advances in Osteosarcoma: Clinical Perspectives: Past, Present and Future","author":"Bhagat A.","year":"2020"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-017-2981-5"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2015.1077195"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2010.05.025"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27123705"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2007.09.007"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543776.2016.1118060"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12640-023-00657-0"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570159X18666200106152631"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M210810200"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2008.03.003"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-022-03377-2"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.117590"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuro.2016.10.008"},{"issue":"4","key":"e_1_2_10_19_1","first-page":"3053","volume":"19","author":"Zhang L.","year":"2019","journal-title":"Mol. 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