{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T04:21:49Z","timestamp":1791260509020,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,7,27]],"date-time":"2024-07-27T00:00:00Z","timestamp":1722038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Lung cancer is known as the most common cancer. Although the <jats:italic>Ramucirumab<\/jats:italic> antibody is a second\u2010line treatment for lung cancer, the high interstitial fluid pressure limits the antibody's performance. In this way, <jats:italic>Imatinib<\/jats:italic> is a chemotherapeutic drug to reduce the interstitial fluid pressure. Up to now, unfortunately, both Ramucirumab and imatinib have not been reported in one nanosystem for cancer therapy. To fulfill this shortcoming, this paper aims to design a chitosan nanocarrier that loads imatinib and attaches to Ramucirumab for selective bonding to A549. Therefore, this paper aims to develop a polymeric nanosystem for non\u2010small cell lung cancer (NSCLC) treatment. In first, the chitosan polyethylene glycol nanoparticle is synthesized, loaded with <jats:italic>imatinib<\/jats:italic>, and then targeted using <jats:italic>Ramucirumab<\/jats:italic>. Afterwards, the CS\u2010PEG\u2010Ab\u2010Im by FTIR, TEM, DLS, zeta potential, and TGA techniques are characterized. The size of CS\u2010PEG\u2010Ab\u2010Im was 25\u201330\u2009nm, its surface charge was 13.1\u2009mV, and the shape of CS\u2010PEG\u2010Ab\u2010Im was nearly spherical and cylindrical. The therapeutic potential of CS\u2010PEG\u2010Ab\u2010Im was assessed using the A549 cell line. According to the obtained results, the cell viability was 48% after 48\u2009h of treatment of A549 cells using the IC50 concentration of CS\u2010PEG\u2010Ab\u2010Im (100 <jats:italic>nanomolar<\/jats:italic>). Moreover, the apoptosis and cell cycle arrest percentages were increased by 3 and 6 times, respectively, as compared to free <jats:italic>imatinib<\/jats:italic>. Furthermore, the release rate of <jats:italic>imatinib<\/jats:italic> from CS\u2010PEG\u2010Ab\u2010Im in an acidic medium was 17% during 1\u2009h, indicating five times the <jats:italic>imatinib<\/jats:italic> release in the natural medium. Eventually, the result of flow cytometry indicates the more apoptotic effect of nanosystem to free imatinib and CS\u2010PEG\u2010Ab. Besides, cell arresting result exhibits the CS\u2010PEG\u2010Ab\u2010Im and causes cell arrested at G1 by %8.17. Thus, it can be concluded that CS\u2010PEG\u2010Ab\u2010Im can be an ideal nanosystem in NSCLC treatment.<\/jats:p>","DOI":"10.1002\/jbt.23787","type":"journal-article","created":{"date-parts":[[2024,7,27]],"date-time":"2024-07-27T18:45:30Z","timestamp":1722105930000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Synthesis of chitosan polyethylene glycol antibody complex for delivery of <i>Imatinib<\/i> in lung cancer cell lines"],"prefix":"10.1002","volume":"38","author":[{"given":"Mehrdad","family":"Kamali","sequence":"first","affiliation":[{"name":"Department of Biology, Science and Research Branch Islamic Azad University Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanieh","family":"Jafari","sequence":"additional","affiliation":[{"name":"Department of Biology, Science and Research Branch Islamic Azad University Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Taati","sequence":"additional","affiliation":[{"name":"Department of Life Science Engineering, Faculty of New Sciences and Technologies University of Tehran Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javad","family":"Mohammadnejad","sequence":"additional","affiliation":[{"name":"Department of Life Science Engineering, Faculty of New Sciences and Technologies University of Tehran Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1104-4626","authenticated-orcid":false,"given":"Amin","family":"Daemi","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine Cukurova University Adana Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-05783-5"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.2c04078"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-05729-x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.105"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/1061186X.2013.837469"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/1061186X.2015.1051049"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25163731"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2019.07.168"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eurpolymj.2012.12.009"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics9040053"},{"key":"e_1_2_8_12_1","first-page":"467","volume":"15","author":"Sanna V.","year":"2014","journal-title":"Int. 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