{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T15:14:16Z","timestamp":1789398856288,"version":"build-2803163510"},"reference-count":52,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T00:00:00Z","timestamp":1724630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Testosterone derived from testicular Leydig cells (LCs) is important for male sheep, and the testis is susceptible to external temperature. The present study aimed to explore the alleviating effect of selenomethionine (Se\u2010Met) on heat\u2010induced injury in Hu sheep LCs. Isolated LCs were exposed to heat (41.5\u00b0C, heat exposure, HE) or not (37\u00b0C, nonheat exposure, NE), and cells in NE and HE were treated with 0 (C) or 8\u2009\u03bcmol\/L (S) Se\u2010Met for 6\u2009h. Cell viability, testosterone level, and the expression of <jats:italic>GPX1<\/jats:italic>, <jats:italic>HSD3B<\/jats:italic>, apoptosis\u2010related genes and p38 mitogen\u2010activated protein kinase (p38MAPK)\/heat shock protein beta\u20101 (HSPB1) pathway were examined. The results showed that Se\u2010Met increased <jats:italic>GPX1<\/jats:italic> expression (NE\u2010S vs. NE\u2010C: 2.28\u2010fold; HE\u2010S vs. HE\u2010C: 2.36\u2010fold, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) and alleviated heat\u2010induced decrease in cell viability (HE\u2010S vs. HE\u2010C: 1.41\u2010fold; HE\u2010C vs. NE\u2010C: 0.61\u2010fold, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01), although the viability was still lower than that in the NE\u2010C cells (HE\u2010S vs. NE\u2010C: 0.85\u2010fold) and Se\u2010Met\u2010treated cells (HE\u2010S vs. NE\u2010S: 0.81\u2010fold). Se\u2010Met relieved heat\u2010induced decrease in testosterone level (HE\u2010S vs. HE\u2010C: 1.84\u2010fold, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) and <jats:italic>HSD3B<\/jats:italic> expression (HE\u2010S vs. HE\u2010C: 1.67\u2010fold, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Se\u2010Met alleviated heat\u2010induced increase in Bcl2\u2010associated protein X\u00a0(BAX) expression (HE\u2010C vs. HE\u2010S: 2.4\u2010fold, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), and decrease in B\u2010cell lymphoma\u20102\u00a0(BCL2) expression (HE\u2010S vs. HE\u2010C: 2.62\u2010fold, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), resulting in increased <jats:italic>BCL2<\/jats:italic>\/<jats:italic>BAX<\/jats:italic> ratio in the HE\u2010S cells (HE\u2010S vs. HE\u2010C: 5.24\u2010fold, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Furthermore, Se\u2010Met alleviated heat\u2010induced activation of p\u2010p38MAPK\/p38MAPK (HE\u2010C vs. HE\u2010S: 1.79\u2010fold, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) and p\u2010HSPB1\/HSPB1 (HE\u2010C vs. HE\u2010S: 2.72\u2010fold, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). In conclusion, p38MAPK\/HSPB1 might be involved in Se\u2010Met\u2010mediated alleviation of heat\u2010induced cell apoptosis, cell viability and testosterone secretion impairments in sheep LCs.<\/jats:p>","DOI":"10.1002\/jbt.23826","type":"journal-article","created":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T05:50:36Z","timestamp":1724737836000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["p38MAPK\/HSPB1 is involved in the regulatory effects of selenomethionine on the apoptosis, viability and testosterone secretion of sheep Leydig cells exposed to heat"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-2514-3737","authenticated-orcid":false,"given":"Yinying","family":"Xu","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Xia","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanli","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dagan","family":"Mao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/iox097"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-020-02475-3"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/83.6.889"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00484-003-0194-y"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:TROP.0000042868.76914.a9"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-018-1642-3"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2021.112387"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2016.09.003"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/1687657"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani9100720"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0240071"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-7-120"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00045-9"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2014.03.016"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2017.01.022"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.40.25602"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2020.105169"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M603622200"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E22-02-0057"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0068978"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2020.05.016"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-17-0016"},{"key":"e_1_2_9_24_1","first-page":"6","volume":"13","author":"Ren Z.","year":"2022","journal-title":"Front. 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