{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T19:32:15Z","timestamp":1789241535718,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,8,31]],"date-time":"2024-08-31T00:00:00Z","timestamp":1725062400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Arsenic (As) is a highly toxic metal and one of the main factors in cancer development through oxidative stress and production of reactive oxygen species. Prior research has demonstrated melatonin's potential as a free radical scavenger. Raf kinase inhibitory protein (RKIP) is an important regulator of intracellular signaling pathways that has been linked to various types of cancer. The aim of this research was to explore the influence of melatonin's antioxidant properties on the expression of the protein RKIP and the antioxidant status of liver tissue in rats that were exposed to arsenic. Thirty two male Wistar rats were divided into four groups of eight, including control, melatonin\u2010treated (20\u2009mg\/Kg of melatonin), sodium arsenite\u2010treated (5.5\u2009mg\/Kg of sodium arsenite), and melatonin + sodium arsenite\u2010treated groups (combination) for 4 weeks. The expression level of protein RKIP was measured by Western blot, and malondialdehyde (MDA) content of the liver as well as the activities of antioxidant enzymes were measured. The data analyzed using one\u2010way ANOVA (significance level of <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) and GraphPad Prism (9) software. Sodium arsenite treatment led to a significant decrease in RKIP protein expression and antioxidant enzyme activity, and an increase in liver MDA levels (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). Conversely, melatonin treatment in the combination group resulted in a significant increase in RKIP protein expression and antioxidant enzyme activity and a decrease in liver MDA levels (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). These findings suggest that melatonin can attenuate oxidative damage caused by arsenic in liver cells by enhancing RKIP protein expression and antioxidant enzyme activity.<\/jats:p>","DOI":"10.1002\/jbt.23835","type":"journal-article","created":{"date-parts":[[2024,8,31]],"date-time":"2024-08-31T14:20:02Z","timestamp":1725114002000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Melatonin alleviates arsenic\u2010induced liver injury by regulating protein RKIP and enhancing antioxidant defencse mechanisms"],"prefix":"10.1002","volume":"38","author":[{"given":"Soheil","family":"Rezvankhah","sequence":"first","affiliation":[{"name":"Department of Biology Higher Education Institute of Rab\u2010Rashid Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7827-9713","authenticated-orcid":false,"given":"Felor","family":"Zargari","sequence":"additional","affiliation":[{"name":"Department of Medical Science Marand Branch, Islamic Azad University Marand Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5079-5655","authenticated-orcid":false,"given":"Rasoul","family":"Sharifi","sequence":"additional","affiliation":[{"name":"Department of Biology Tabriz Branch, Islamic Azad University Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,31]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-020-02889-2"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2015.70"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13246247"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/8414047"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2021.115738"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.22291"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-021-06669-3"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1339304"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2020.105210"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14205070"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12652"},{"key":"e_1_2_8_13_1","first-page":"44","volume":"36","author":"Zargari F.","year":"2014","journal-title":"Med. 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