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However, the exact mechanism of these toxicities has still not been fully deciphered. Additionally, studies have reported the toxicological effects at far low doses to the generally considered no\u2010observed\u2010adverse\u2010effect level (NOAEL) dose. The present study investigates the effects of a sub\u2010acute (28 days) exposure to BPA (10, 50 and 100\u2009mg\/kg\/day) in adult male mice on various hormones levels, sperm motility, sperm count, functional integrity of sperm plasma membrane, testicular histological changes, oxidative stress markers and DNA damage. The key proteome signatures were quantified by LC\u2010MS\/MS analysis using Orbitrap Fusion Lumos Tribrid Mass Spectrometer equipped with nano\u2010LC Easy\u2010nLC 1200. Data suggest that the BPA exposure in all doses (below\/above NOAEL dose) have greatly impacted the hormone levels, sperm parameters (sperm count, motility and membrane integrity) and testicular histology. Mass spectrometry\u2010based proteomics data suggested for 1352 differentially expressed proteins (DEPs; 368 upregulated, 984 downregulated) affecting biological process, cellular component, and molecular functions. Specifically searched male reproductive function related proteins suggested a complex network where 46 potential proteins regulating spermatogenesis, sperm structure, activity and membrane integrity while tackling oxidative stress responses were downregulated. These potential biomarkers could shed some more light on our current understanding of the reproductive toxicological effects of BPA and may lead to exploration of novel interventions strategies against these targets for male infertility.<\/jats:p>","DOI":"10.1002\/jbt.23862","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T09:34:25Z","timestamp":1727256865000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Sub\u2010acute bisphenol A exposure induces proteomic alterations and impairs male reproductive health in mice"],"prefix":"10.1002","volume":"38","author":[{"given":"Shiv K.","family":"Yadav","sequence":"first","affiliation":[{"name":"ICMR\u2010National Institute of Occupational Health (NIOH) Ahmedabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arvind","family":"Kumar","sequence":"additional","affiliation":[{"name":"International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bal G.","family":"Yadav","sequence":"additional","affiliation":[{"name":"Institute of Plant and Microbial Biology Agricultural Technology Building Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vandana","family":"Bijalwan","sequence":"additional","affiliation":[{"name":"ICMR\u2010National Institute of Occupational Health (NIOH) Ahmedabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh","family":"Yadav","sequence":"additional","affiliation":[{"name":"ICMR\u2010National Institute for Implementation Research on Non\u2010Communicable Disease (NIIRNCD) Jodhpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gajanan P.","family":"Patil","sequence":"additional","affiliation":[{"name":"ICMR\u2010National Institute of Occupational Health (NIOH) Ahmedabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9193-0849","authenticated-orcid":false,"given":"Kamalesh","family":"Sarkar","sequence":"additional","affiliation":[{"name":"ICMR\u2010National Institute of Occupational Health (NIOH) Ahmedabad India"},{"name":"ICMR\u2010National Institute of Cholera &amp; 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