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The biotransfomation of MeAN to CN<jats:sup>\u2212<\/jats:sup> was characterized with respect to time, microsomal protein concentration, pH, and temperature. Metabolism of MeAN was increased in microsomes obtained from phenobarbital\u2010treated rats (310% of control) and decreased with CoCl<jats:sub>2<\/jats:sub> and SKF 525 A treatments (55% and 61%, respectively). Addition of the epoxide hydratase inhibitor, 1,1,1\u2010trichloropropane 2,3\u2010oxide, decreased the formation of CN<jats:sup>\u2212<\/jats:sup> from MeAN. Addition of glutathione, cysteine, D\u2010penicillamine, and 2\u2010mercaptoethanol enhanced the released of CN<jats:sup>\u2212<\/jats:sup> from MeAN. These findings indicate that MeAN is metabolized to CN<jats:sup>\u2212<\/jats:sup> via a cytochrome P\u2010450\u2010dependent mixed\u2010function oxidase system.<\/jats:p>","DOI":"10.1002\/jbt.2570050205","type":"journal-article","created":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T01:35:14Z","timestamp":1161135314000},"page":"109-114","source":"Crossref","is-referenced-by-count":7,"title":["Metabolism of methacrylonitrile to cyanide: In vitro studies"],"prefix":"10.1002","volume":"5","author":[{"given":"Mohammed Y. 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