{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T13:10:54Z","timestamp":1698066654185},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,6,8]],"date-time":"2006-06-08T00:00:00Z","timestamp":1149724800000},"content-version":"vor","delay-in-days":5486,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Biochem. Toxicol."],"published-print":{"date-parts":[[1991,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, we have characterized the covalent binding of [<jats:sup>14<\/jats:sup>C]\u20102\u2010chloro\u20104\u2010acetotoluidide (CAT) radioactivity to microsomes of starling liver and kidney. The maximal velocity (V<jats:sub>max<\/jats:sub>) of covalent binding and apparent Michaelis constant (K<jats:sub>m<\/jats:sub>) for both tissues were similar. The V<jats:sub>max<\/jats:sub> for liver and kidney were 52.8 and 68.9 pmol\/min\/mg protein, and the apparent K<jats:sub>ms<\/jats:sub> were 0.54 and 0.87 mM, respectively. The covalent binding of radioactivity to heat\u2010denatured microsomes of liver and kidney was reduced by 62% and 15%, respectively. Incubation at 0\u00b0C reduced the binding by 80% to liver and 70% to kidney microsomes. Absence of nicotinamide adenine dinucleotide phosphate (NADP) and molecular O<jats:sub>2<\/jats:sub> reduced the binding to liver microsomes by 36 and 53%, as opposed to 28% increase and 26% decrease in binding to kidney microsomes, respectively. Inducers of cytochrome P<jats:sub>450<\/jats:sub> monooxygenase (P<jats:sub>450<\/jats:sub>), phenobarbital, and 3\u2010methylcholanthrene (3\u2010MC), had opposite effects on the covalent binding of [<jats:sup>14<\/jats:sup>C]\u2010CAT radioactivity to hepatic and renal microsomes. Phenobarbital increased the binding to hepatic microsomes by 100% and had no effect on binding to renal microsomes. 3\u2010MC, on the other hand, increased the binding to kidney microsomes by threefold and had no effect on the binding to hepatic microsomes. SKF 525A, an inhibitor of P<jats:sub>450<\/jats:sub>, inhibited the binding to hepatic microsomes by 60% at 0.5 mM but failed to have any effect on binding to renal microsomes. \u03b1\u2010Naphthoflavone, another inhibitor of P<jats:sub>450<\/jats:sub>, had no effect on the covalent binding of [<jats:sup>14<\/jats:sup>C]\u2010CAT radioactivity to microsomes of either tissue. Addition of cysteine and glutathione in the incubation mixture inhibited the covalent binding of radioactivity to both liver and kidney microsomes in a concentration\u2010dependent manner. It was concluded that hepatic and renal microsomes of starling behave differently with respect to the effects of P<jats:sub>450<\/jats:sub> inducers and inhibitors, heat denaturation, NADPH dependence, and presence of molecular oxygen on bioactivation of [<jats:sup>14<\/jats:sup>C]\u2010CAT and its subsequent covalent binding to microsomal proteins.<\/jats:p>","DOI":"10.1002\/jbt.2570060208","type":"journal-article","created":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T01:40:43Z","timestamp":1161135643000},"page":"137-145","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of covalent binding of [<sup>14<\/sup>C]\u20102\u2010chloro\u20104\u2010acetotoluidide to microsomes of starling liver and kidney"],"prefix":"10.1002","volume":"6","author":[{"given":"Shri N.","family":"Giri","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Siegel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,6,8]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4800(78)90083-7"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-008X(71)80004-2"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(72)90210-3"},{"key":"e_1_2_1_5_2","unstructured":"A.Apostolou(1969). 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