{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:39:37Z","timestamp":1759941577701},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,6,9]],"date-time":"2006-06-09T00:00:00Z","timestamp":1149811200000},"content-version":"vor","delay-in-days":4391,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Biochem. Toxicol."],"published-print":{"date-parts":[[1994,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The mechanism by which organo\u2010phosphorus\u2010induced delayed polyneuropathy is induced relates to the specific inhibition and subsequent modification (\u201caging\u201d) of a protein known as neuropathy target esterase (NTE), operatively defined as paraoxon\u2010resistant and mipafox\u2010sensi\u2010tive phenyl valerate (PV) esterase activity. This protein has fundamentally been investigated in hen brain, the latter being the habitually employed OPIDP study model. In the present article, a partial characterization is made of the NTE and other related PV esterases in the bovine adrenal medulla and brain; NTE sensitivity to the neurotoxic or\u2010ganophosphorus compound mipafox is investigated, and its subcellular distribution is studied. The NTE activity of the adrenal medulla was found to be the highest of those among the tissues studied to date (5000 \u00b1 1400 mU\/g tissue; \u00b1 SD, <jats:italic>n<\/jats:italic> = 12). This activity represented 93% of the PV esterase activity resistant to 40 \u03bcm paraoxon in the par\u2010ticulate fraction of the adrenal medulla and approximately 50% of total PV esterase activity. In the bovine brain, these proportions were 72 and 26%, respectively, <jats:italic>i.e.<\/jats:italic>, similar to those described in hen brain. The mipafox inhibition curve of PV esterase activity resistant to 40\u03bcM paraoxon in the particulate fraction of the adrenal medulla suggests that NTE activity fundamentally comprises a mipafox\u2010sensitive component with an <jats:italic>I<\/jats:italic> <jats:sub>50<\/jats:sub> of 6.39 \u03bcM at 30 minutes, which is similar to the value reported in hen brain. NTE activity in the bovine adrenal medulla is almost exclusively limited to the particulate fraction, the microsomal fraction, plasma membrane, and chromaffin granule\u2010enriched fractions being the highest in terms of specific activity. On the contrary, the mitochondria\u2010enriched fraction was very poor in such activity. In bovine brain, most NTE activity was likewise limited to the particulate fraction.<\/jats:p>","DOI":"10.1002\/jbt.2570090306","type":"journal-article","created":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T02:04:40Z","timestamp":1161137080000},"page":"145-152","source":"Crossref","is-referenced-by-count":18,"title":["Partial characterization of neuropathy target esterase and related phenyl valerate esterases from bovine adrenal medulla"],"prefix":"10.1002","volume":"9","author":[{"given":"M. A.","family":"Sogorb","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Viniegra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. A.","family":"Reig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Vilanova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,6,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1850739"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0300-483X(88)90181-3"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0009-2797(93)90065-7"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.3109\/10408449209089884"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(82)90351-3"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2090817"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(92)90240-S"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4274(92)90053-M"},{"key":"e_1_2_1_10_2","first-page":"1","article-title":"Development of catecholamine storing cells in human fetal paraganglia and adrenal medulla. A histochemical and electromicroscopical Study","volume":"368","author":"Hervonen A.","year":"1971","journal-title":"Physiologica Scandinavica"},{"key":"e_1_2_1_11_2","first-page":"12","article-title":"Functional and morphological characterization of isolated bovina adrenal medullary cells","volume":"76","author":"Fendvick E. M.","year":"1978","journal-title":"J. Cellular Biochem."},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/neu.480240206"},{"key":"e_1_2_1_13_2","first-page":"174","article-title":"Control of exocytosis in adrenal chromaffin cells","volume":"1071","author":"Burgogne R. D.","year":"1991","journal-title":"Biochem. Biophys. Acta"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1080\/15287399309531705"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(92)90286-2"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1990.tb12082.x"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00293860"},{"key":"e_1_2_1_18_2","volume-title":"Data Reductions and Error Analysis for the Physical Sciences","author":"Bevington P. R.","year":"1969"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(85)90380-1"},{"key":"e_1_2_1_21_2","doi-asserted-by":"crossref","unstructured":"V.Carrera N.Diaz\u2010Alejo M. A.Sogorb J. L.Vi\u2010cedo E.Vilano(1993). \u201cIn vivo\u201d inhibition by mipafox of soluble and particulate forms of organo\u2010phosphorus neuropathy target esterase (NTE) in hen sciatic nerve.Toxicol. Lett. in press.","DOI":"10.1016\/0378-4274(94)90197-X"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1993.tb07455.x"},{"key":"e_1_2_1_23_2","first-page":"141","article-title":"The target for initiation of delayed neurotoxicity by organophosphorus esters: biochemical studies and toxicological applications","volume":"4","author":"Johnson M. K.","year":"1982","journal-title":"Rev. Biochem. Toxicol."},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02034943"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02307272"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(87)90630-7"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4274(87)90185-8"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0272-0590(91)90140-Y"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1979.tb00391.x"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1080\/03601238009372175"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1990.tb03133.x"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(91)90567-O"},{"key":"e_1_2_1_33_2","first-page":"63","article-title":"Inhibitor characteristics of native, solubilized, and lipid reconstituted neurotoxic esterase","volume":"51","author":"Davis C. S.","year":"1980","journal-title":"Toxicol. Lett."},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1980.tb11208.x"}],"container-title":["Journal of Biochemical Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbt.2570090306","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.2570090306","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T21:12:15Z","timestamp":1698181935000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.2570090306"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,6]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1994,6]]}},"alternative-id":["10.1002\/jbt.2570090306"],"URL":"https:\/\/doi.org\/10.1002\/jbt.2570090306","archive":["Portico"],"relation":{},"ISSN":["0887-2082","1522-7146"],"issn-type":[{"value":"0887-2082","type":"print"},{"value":"1522-7146","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,6]]}}}