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These cells undergo adaptive changes through the expression of hypoxia\u2010inducible factor\u20101alpha (HIF\u20101\u03b1) which is regulated by factor inhibiting HIF\u20101\u03b1 (FIH\u20101). Considering this, we hypothesized that the chemical activation of FIH\u20101 would inhibit the hypoxic activity of HIF\u20101\u03b1 in mammary gland carcinoma. A library of 67,609 chemical compounds was virtually screened against FIH\u20101 based on Lipinski's rule from the ZINC database. The BBAP\u20108 has been selected based on an excellent docking score (\u22128.352\u2009Kcal\/mol), favorable ADMET, and potential FIH\u20101 activator profile. Further, its in\u2010vitro cytotoxicity and apoptotic activity were scrutinized against MCF\u20107 cells and in\u2010vivo activity against 7,12\u2010dimethylbenz[a]anthracene (DMBA) induced mammary gland carcinoma in Wistar rats. It exhibited significant cytotoxicity (IC50\u2009=\u200916.59\u2009\u00b1\u20090.49\u2009\u03bcM) and activated apoptosis when scrutinized through DAPI, AO\/EB, and JC\u20101 staining. Also, oral administration of BBAP\u20108 restored hemodynamic changes, normalized tissue architecture, and corrected metabolic abnormalities. The western blot analysis and mRNA expression analysis validated that BBAP\u20108 has the potential to activate FIH\u20101 with the downregulation of GLUT\u20101, VEGF, and Twist\u20101. Moreover, BBAP\u20108 fostered apoptosis, when evaluated through BCL\u20102, BAX, Caspase\u20108, and Caspase\u20103. Based on research findings, this implies that BBAP\u20108 activates FIH\u20101 and can be effective in chemotherapeutic treatment of mammary gland carcinoma.<\/jats:p>","DOI":"10.1002\/jbt.70016","type":"journal-article","created":{"date-parts":[[2024,10,19]],"date-time":"2024-10-19T05:12:50Z","timestamp":1729314770000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A novel quinazoline derivative exhibits potent anticancer cytotoxicity via apoptosis and inhibition of angiogenesis in DMBA\u2010induced mammary gland carcinoma"],"prefix":"10.1002","volume":"38","author":[{"given":"Soniya","family":"Rani","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences Babasaheb Bhimrao Ambedkar University (A Central University) Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rimjhim","family":"Trivedi","sequence":"additional","affiliation":[{"name":"Department of Advanced Spectroscopy and Imaging, Centre of Biomedical Research Sanjay Gandhi Post Graduate Institute of Medical Sciences Lucknow India"},{"name":"Academy of Scientific and Innovative Research Ghaziabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohd. Nazam","family":"Ansari","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, College of Pharmacy Prince Sattam Bin Abdulaziz University Al\u2010Kharj Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulaziz S.","family":"Saeedan","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, College of Pharmacy Prince Sattam Bin Abdulaziz University Al\u2010Kharj Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dinesh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Advanced Spectroscopy and Imaging, Centre of Biomedical Research Sanjay Gandhi Post Graduate Institute of Medical Sciences Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunil Kumar","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences United Institute of Pharmacy, United Group of Institutions Prayagraj India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alok","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences United Institute of Pharmacy, United Group of Institutions Prayagraj India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manjari","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences Assam University Silchar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1649-5434","authenticated-orcid":false,"given":"Gaurav","family":"Kaithwas","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences Babasaheb Bhimrao Ambedkar University (A Central University) Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,18]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14123021"},{"key":"e_1_2_11_3_1","first-page":"199","volume-title":"Hormone Related Cancer Mechanistic and Nanomedicines: Challenges and Prospects","author":"Mustafa G.","year":"2023"},{"key":"e_1_2_11_4_1","first-page":"123","author":"Minassian L. 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