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The expression levels of HOXA10\/long noncoding RNA (lncRNA) PTPRG antisense RNA 1 (PTPRG\u2010AS1)\/ubiquitin\u2010specific peptidase 1 (USP1) in NPC tissues and cells were determined. Cell proliferation was evaluated. The enrichment of HOXA10 on the PTPRG\u2010AS1 promoter was determined. The binding of PTPRG\u2010AS1, HuR, and USP1 to each other was analyzed via RNA immunoprecipitation. USP1 mRNA stability was determined after actinomycin D treatment. The role of the PTPRG\u2010AS1\/USP1 axis in NPC cell proliferation was analyzed in combined experiments. The role of HOXA10 in vivo was confirmed in xenograft tumor models. The results revealed that HOXA10 was overexpressed in NPC. HOXA10 downregulation reduced NPC cell proliferation. PTPRG\u2010AS1 and USP1 were upregulated in NPC. HOXA10 bound to the PTPRG\u2010AS1 promoter to increase PTPRG\u2010AS1 expression, and the binding of PTPRG\u2010AS1 to HuR increased USP1 expression. PTPRG\u2010AS1 or USP1 overexpression attenuated the inhibitory effects of HOXA10 downregulation on NPC cell proliferation. HOXA10 downregulation inhibited in vivo NPC proliferation through the PTPRG\u2010AS1\/USP1 axis. In conclusion, HOXA10 facilitates NPC cell proliferation in vitro and in vivo through the PTPRG\u2010AS1\/USP1 axis.<\/jats:p>","DOI":"10.1002\/jbt.70025","type":"journal-article","created":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T09:59:03Z","timestamp":1729763943000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Mechanism of HOXA10 in nasopharyngeal carcinoma cell proliferation through the PTPRG\u2010AS1\/USP1 axis"],"prefix":"10.1002","volume":"38","author":[{"given":"He","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Pathology Affiliated Hospital of Jining Medical University Jining China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang","family":"Deng","sequence":"additional","affiliation":[{"name":"Forensic Laboratory of Material Evidence Identification Department Jinan Municipal Bureau of Public Security Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3696-0764","authenticated-orcid":false,"given":"Yue","family":"Xi","sequence":"additional","affiliation":[{"name":"Department of Pathology Heze Municipal Hospital Heze China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1259\/bjr.20190244"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-20-1702"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2021.12775"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(19)30956-0"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2020.112758"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics13162641"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/19458924221100960"},{"issue":"3","key":"e_1_2_9_9_1","first-page":"836","volume":"45","author":"Li H.","year":"2020","journal-title":"Int. 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