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Chemotherapy resistance in TNBC is largely driven by the abnormal activation of epithelial\u2010to\u2010mesenchymal transition (EMT) and the associated cancer stem cell\u2010like characteristics. The combination of multiple chemotherapeutic drugs has shown promise as a treatment approach for TNBC. This study evaluates the efficacy of a novel combination therapy involving the anti\u2010inflammatory drug Budesonide and Salinomycin, which targets cancer stem cells. Co\u2010administration of Budesonide and Salinomycin demonstrated a synergistic effect in inhibiting TNBC cell growth by activating the intrinsic apoptosis pathway. It induced a 2\u2010 to 3\u2010fold increase in intracellular reactive oxygen species (ROS) generation and a 25%\u201330% rise in mitochondrial membrane depolarization. Additionally, extensive signaling studies revealed that the co\u2010treatment specifically targeted multiple signaling nodes, limiting downstream crosstalk. The combination also enhanced autophagic activity by inhibiting the AKT\/mTOR pathway and reduced cell migration and stemness by suppressing the EMT process. Therefore, the combination of Budesonide and Salinomycin offers a novel therapeutic approach for TNBC.<\/jats:p>","DOI":"10.1002\/jbt.70045","type":"journal-article","created":{"date-parts":[[2024,11,11]],"date-time":"2024-11-11T12:05:37Z","timestamp":1731326737000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Synergistic Effect of Salinomycin With Budesonide on TNBC Regression via EMT Reversal and Autophagy Induction"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8884-8812","authenticated-orcid":false,"given":"Shilpi","family":"Sarkar","sequence":"first","affiliation":[{"name":"Department of Biosciences and Bioengineering Indian Institute of Technology Guwahati Guwahati Assam India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5019-0936","authenticated-orcid":false,"given":"Siddhartha 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