{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T03:29:20Z","timestamp":1782358160682,"version":"3.54.5"},"reference-count":39,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T00:00:00Z","timestamp":1732579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T00:00:00Z","timestamp":1732579200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Cell\u2010cell adhesion between thyroid tumor cells and pulmonary endothelial cells plays a critical role in the development of lung metastases from primary thyroid cancer. Dabrafenib, a selective inhibitor for B\u2010RAF kinase, has been approved for cancer treatment. However, its effects on pulmonary metastases originating from primary thyroid cancer remain unclear. In this study, we demonstrate that conditioned medium (CM) from the thyroid cancer SW579 cell line significantly elevated the expression of pro\u2010inflammatory cytokines HMGB\u20101, IL\u20101\u03b2, and MCP\u20101 in human pulmonary microvascular endothelial cells (HPMECs), which was notably reduced by Dabrafenib. Additionally, exposure to the thyroid cancer SW579 CM increased the expression of endothelial adhesion molecules VCAM\u20101 and ICAM\u20101, as well as the adhesion of thyroid cancer SW579 cells to HPMECs, both of which were prevented by Dabrafenib. We also found that Dabrafenib mitigated oxidative stress induced by SW579 CM, as evidenced by increased glutathione peroxidase (GSH\u2010Px) activity and reduced malondialdehyde (MDA) levels. Further investigation revealed that Dabrafenib's beneficial effects were mediated through the inhibition of Egr\u20101, and overexpression of Egr\u20101 reversed Dabrafenib's protective effect on the adhesion of thyroid cancer cells to HPMECs. Based on these results, we propose that Dabrafenib may have the potential to prevent pulmonary metastases of thyroid cancer cells.<\/jats:p>","DOI":"10.1002\/jbt.70060","type":"journal-article","created":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T05:56:22Z","timestamp":1732600582000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Dabrafenib Inhibits Egr\u20101\u2010Mediated Adhesion of Thyroid Cancer Cells to Pulmonary Microvascular Endothelium"],"prefix":"10.1002","volume":"38","author":[{"given":"Yanchao","family":"Qin","sequence":"first","affiliation":[{"name":"Department of Head and Neck Surgery Shanxi Provincial Cancer Hospital Taiyuan China"},{"name":"Department of Head and Neck Surgery Shanxi Hospital Affiliated to Cancer Hospital Chinese Academy of Medical Sciences Taiyuan China"},{"name":"Department of Head and Neck Surgery Cancer Hospital Affiliated to Shanxi Medical University Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Head and Neck Surgery Shanxi Provincial Cancer Hospital Taiyuan China"},{"name":"Department of Head and Neck Surgery Shanxi Hospital Affiliated to Cancer Hospital Chinese Academy of Medical Sciences Taiyuan China"},{"name":"Department of Head and Neck Surgery Cancer Hospital Affiliated to Shanxi Medical University Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuesong","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Head and Neck Surgery Shanxi Provincial Cancer Hospital Taiyuan China"},{"name":"Department of Head and Neck Surgery Shanxi Hospital Affiliated to Cancer Hospital Chinese Academy of Medical Sciences Taiyuan China"},{"name":"Department of Head and Neck Surgery Cancer Hospital Affiliated to Shanxi Medical University Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7063-9378","authenticated-orcid":false,"given":"Dongguang","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Head and Neck Surgery Shanxi Provincial Cancer Hospital Taiyuan China"},{"name":"Department of Head and Neck Surgery Shanxi Hospital Affiliated to Cancer Hospital Chinese Academy of Medical Sciences Taiyuan China"},{"name":"Department of Head and Neck Surgery Cancer Hospital Affiliated to Shanxi Medical University Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecl.2018.10.002"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(23)00020-X"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.1099280"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/endocr\/bqad118"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000034733"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1417273112"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.834472"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.28046"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10238-024-01490-1"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-54490-3_14"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.semdp.2015.02.010"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/1060028013513009"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-23-0046"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-50369-w"},{"key":"e_1_2_9_16_1","first-page":"1593","article-title":"Simvastatin Reduces Tumor Cell Adhesion to Human Peritoneal Mesothelial Cells by Decreased Expression of VCAM\u20101 and \u03b21 Integrin","volume":"39","author":"Wagner B. J.","year":"2011","journal-title":"International Journal of Oncology"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-022-02207-y"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2018.01.002"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612827666210303143442"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.33486"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-022-01057-0"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2023.117487"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-022-00923-5"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.2MR0220-549R"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19041057"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2018.08.021"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01078"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10151-004-0115-z"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.1052672"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-011-0984-2"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmz105"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-57613-8_4"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.114775"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2015.01.002"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2021.104179"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S76114"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2019.102343"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2019.01.008"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032-019-1333-6"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2020.106224"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.70060","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbt.70060","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.70060","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T09:00:35Z","timestamp":1774342835000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.70060"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,26]]},"references-count":39,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1002\/jbt.70060"],"URL":"https:\/\/doi.org\/10.1002\/jbt.70060","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,26]]},"assertion":[{"value":"2024-06-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70060"}}