{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T00:17:28Z","timestamp":1770423448999,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,2,26]],"date-time":"2025-02-26T00:00:00Z","timestamp":1740528000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Posterior capsular opacification (PCO) is the most common complication after cataract surgery characterized by hyperproliferation, migration and epithelial\u2010mesenchymal transition (EMT) in residual lens epithelial cells (LECs). Curcumin is a polyphenol compound possessing diverse pharmacological properties. Here, we investigated the functions and its potential mechanisms of curcumin in PCO using transforming growth factor beta2 (TGF\u2010\u03b22)\u2010treated LECs. Cell phenotypes were analyzed using MTT, 5\u2010thynyl\u20102\u2032\u2010deoxyuridine (EdU), transwell, and scratch assays, respectively. Levels of FGF7 (Fibroblast Growth Factor 7), ZEB1 (Zinc finger E\u2010box binding homeobox 1), and EMT\u2010related proteins were detected by qRT\u2010PCR and western blot analysis. The protein interaction between FGF7 and ZEB1 was validated using Co\u2010immunoprecipitation assay. Curcumin treatment weakened TGF\u2010\u03b22\u2010induced proliferation, migration, invasion and EMT progression in LECs. The expression of FGF7 was boosted by curcumin in LECs. Functionally, FGF7 deficiency suppressed TGF\u2010\u03b22\u2010induced proliferation, migration, invasion and EMT progression in LECs, and could reverse the suppressing action of curcumin on TGF\u2010\u03b22\u2010induced LEC dysfunction. Mechanistically, FGF7 directly interacted with ZEB1, and curcumin could regulate ZEB1 expression via FGF7. Moreover, ZEB1 overexpression could abolish the protective effects of curcumin or FGF7 deficiency on LECs under TGF\u2010\u03b22 stimulation. In conclusion, curcumin protected LECs against TGF\u2010\u03b22\u2010induced enhancement on the proliferation, migration, invasion and EMT process by regulating FGF7\/ZEB1 axis, suggesting a new insight into the application of curcumin in PCO therapy.<\/jats:p>","DOI":"10.1002\/jbt.70191","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T01:49:12Z","timestamp":1741744152000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Curcumin Restrains TGF\u2010\u03b22\u2010Induced Proliferation, Migration, Invasion and EMT in Lens Epithelial Cells by Regulating FGF7\/ZEB1 Axis"],"prefix":"10.1002","volume":"39","author":[{"given":"Jing","family":"He","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Jiujiang City Key Laboratory of Cell Therapy JiuJiang NO.1 People's Hospital Jiujiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0539-6772","authenticated-orcid":false,"given":"Ping","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Jiujiang City Key Laboratory of Cell Therapy JiuJiang NO.1 People's Hospital Jiujiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangjun","family":"Ou","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Jiujiang City Key Laboratory of Cell Therapy JiuJiang NO.1 People's Hospital Jiujiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,2,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pop.2015.05.012"},{"issue":"1","key":"e_1_2_10_3_1","first-page":"58","article-title":"The Evolution of Cataract Surgery","volume":"113","author":"Davis G.","year":"2016","journal-title":"Missouri Medicine"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfo.2014.09.003"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/archophthalmol.2009.3"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10132847"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2020.100905"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11152385"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2021.120061"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2023.156144"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1216708"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11122989"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutres.2014.09.001"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.2174\/0115701808241753231024111800"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.114689"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566524017666171106115655"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-020-03014-2"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1631\/jzus.B1100278"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/6061894"},{"issue":"5","key":"e_1_2_10_20_1","first-page":"722","article-title":"Curcumin Induces Human Lens Epithelial Cell Apoptosis and Cell Cycle Arrest by Inhibiting Wnt\/\u03b2\u2010catenin Signaling Pathway","volume":"41","author":"Zhu M.","year":"2021","journal-title":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/02713683.2021.2021537"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2024.100801"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-024-05085-y"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-019-1603-y"},{"issue":"1","key":"e_1_2_10_25_1","article-title":"Hyaluronic Acid\u2010Mediated Epithelial\u2010Mesenchymal Transition of Human Lens Epithelial Cells via CD44 and TGF\u2010\u03b22","volume":"88","author":"Gui Y.","year":"2024","journal-title":"Arquivos brasileiros de oftalmologia"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12060827"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2021.108572"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201200718"},{"issue":"4","key":"e_1_2_10_29_1","first-page":"249","article-title":"Curcumin: Therapeutical Potential in Ophthalmology","volume":"80","author":"Pescosolido N.","year":"2014","journal-title":"Planta Medica"},{"issue":"21","key":"e_1_2_10_30_1","first-page":"3527","article-title":"An Experimental Study on the Effects of Curcumin on Posterior Capsule Opacification in Young Rabbit Eyes","volume":"124","author":"Wang W. Y.","year":"2011","journal-title":"Chinese Medical Journal"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.2174\/09298665113206660115"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.65240"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19990719)82:2<237::AID-IJC14>3.0.CO;2-Q"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2017.3927"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2022.102468"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2019.03.001"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-023-04831-0"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.04.055"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/8352579"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.70191","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T12:52:19Z","timestamp":1748350339000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.70191"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,26]]},"references-count":38,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1002\/jbt.70191"],"URL":"https:\/\/doi.org\/10.1002\/jbt.70191","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,26]]},"assertion":[{"value":"2024-10-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70191"}}