{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:44:42Z","timestamp":1786977882159,"version":"build-2736575974"},"reference-count":61,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T00:00:00Z","timestamp":1741564800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cylophosphamide (CP)\u2010induced acute cystitis is a debilitating bladder dysfunction commonly observed in cancer patients, primarily resulting from oxidative damage and inflammation in the bladder tissue. Dimethyl fumarate (DMF) is a fumaric acid ester approved for the treatment of multiple sclerosis due to its antioxidant and anti\u2010inflammatory properties. Thus, we aimed to investigate the multiple effects of DMF, involving both its potential synergistic effect with CP on the SH\u2010SY5Y cells and its uroprotective effect on CP\u2010induced acute cystitis. Female Balb\/c mice were orally administrated DMF (100 or 300\u2009mg\/kg\/day) for five consecutive days before a single intraperitoneal (i.p) dose of CP. Mesna was administered 20\u2009min before and at 4\u2009h, 8\u2009h after CP application. Following 24\u2009h of CP injection, bladders were removed for functional, biochemical analysis and evaluation of vesical vascular permeability. SH\u2010SY5Y cell viability was assayed by MTT test. CP markedly decreased carbachol\u2010induced contraction of detrusor strips (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01), which was prevented by the high\u2010dose DMF treatment (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Evans blue dye extravasation was greatly increased in the bladders of cystitis group (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001), which was significantly decreased in DMF\u2010treated mice with cystitis (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01). Total GSH content was decreased (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01) whereas TNF\u2010\u03b1 level was increased (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) in the bladders of cystitis group. High\u2010dose DMF\u2010treated mice showed an increment in total GSH content (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) without any alterations on TNF\u2010\u03b1 levels of the bladders with cystitis. Additionally, combination of different concentrations of CP and DMF exhibited a potent synergistic cytotoxic effect in SH\u2010SY5Y cells. DMF improved CP\u2010induced acute cystitis by partially suppressing oxidative stress and inflammation, while also enhancing the cytotoxic effects of CP.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70212","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T00:39:50Z","timestamp":1741739990000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Dimethyl Fumarate Attenuates Cyclophosphamide\u2010Induced Bladder Damage and Enhances Cytotoxic Activity Against SH\u2010SY5Y Cells"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3284-848X","authenticated-orcid":false,"given":"Elif Nur","family":"Barut","sequence":"first","affiliation":[{"name":"Department of Pharmacology Faculty of Pharmacy, Karadeniz Technical University Trabzon Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se\u00e7kin","family":"Engin","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Faculty of Pharmacy, Karadeniz Technical University Trabzon Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elif","family":"\u00d6z","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Toxicology Ac\u0131badem Mehmet Ali Ayd\u0131nlar University Faculty of Pharmacy \u0130stanbul Turkiye"},{"name":"Department of Pharmaceutical Toxicology Istanbul University Institute of Health Science \u0130stanbul Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3484-2201","authenticated-orcid":false,"given":"Rengin","family":"Reis","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Toxicology Ac\u0131badem Mehmet Ali Ayd\u0131nlar University Faculty of Pharmacy \u0130stanbul Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2016.07.030"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-006-0078-0"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(01)01141-4"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajco.13048"},{"issue":"3","key":"e_1_2_10_6_1","first-page":"199","article-title":"Mechanisms of Hemorrhagic Cystitis","volume":"2","author":"Haldar S.","year":"2014","journal-title":"American Journal of Clinical and Experimental Urology"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2021.10056"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urols.2014.03.001"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-09155-3"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.16140"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.12291"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)68238-7"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X1999001000006"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00280-006-0307-5"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2022.175025"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-016-0496-0"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-019-01229-3"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph13100306"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9040354"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.612981"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1264842"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001813-200502000-00008"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23158681"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b18-00855"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2022.120590"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11255-019-02128-y"},{"key":"e_1_2_10_27_1","article-title":"Evaluating Vascular Hyperpermeability\u2010Inducing Agents in the Skin With the Miles Assay","volume":"136","author":"Brash J. 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