{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T13:08:28Z","timestamp":1792760908234,"version":"4.5.0"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T00:00:00Z","timestamp":1742515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T00:00:00Z","timestamp":1742515200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Chemotherapeutic drugs administered during cancer therapy, may lead to the depletion of ovarian follicles, and subsequent infertility in fertile patients. We aimed to determine toxic effects of bleomycin (BLM) on rat ovary, and to evaluate protective effects of alpha lipoic acid (ALA) on BLM toxicity. The total of 30 adult female rats were split into 4 groups. First, an intramuscular injection (i.m) of BLM (30\u2009mg\/m\n                    <jats:sup>2<\/jats:sup>\n                    ) was administered to BLM and BLM\u2009+\u2009ALA groups except the control and ALA groups on the 1st, 8th and 15th days. The control group received 0.1\u2009mL (i.m) saline on those days. BLM\u2009+\u2009ALA group received ALA (50\u2009mg\/kg) subcutaneously (s.c) for 2 weeks at the same time with BLM injections, and ALA group received ALA s.c for the same period. Ovarian tissues were evaluated by histopathological, stereological, immunohistochemical (StAR, VDAC2, Caspase\u20103, Bcl\u20102, and expression levels) and ELISA (AMH serum levels) methods. The vascular areas and collagen density increased in the medulla, and the volumes of medulla, cortex, and total ovary increased in BLM group, whereas these changes decreased in BLM\u2009+\u2009ALA group. On the other hand, VDAC2 and Caspase\u20103 expressions decreased, StAR and Bcl\u20102 expressions increased in BLM group, whereas VDAC2 and Caspase\u20103 expressions increased, and StAR and Bcl\u20102 expression levels significantly decreased in BLM\u2009+\u2009ALA group. Besides, follicle number and AMH levels decreased in the BLM group, but remarkably increased in the BLM\u2009+\u2009ALA group. We established that ALA may have ameliorative effects on the harmful effects of BLM on ovary.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70230","type":"journal-article","created":{"date-parts":[[2025,3,23]],"date-time":"2025-03-23T09:05:15Z","timestamp":1742720715000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Evaluation of the Protective Effects of Alpha Lipoic Acid on Bleomycin\u2010Induced Ovarian Toxicity"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7695-3049","authenticated-orcid":false,"given":"Nese","family":"Colcimen","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Van Yuzuncu Yil University  Van Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4726-982X","authenticated-orcid":false,"given":"Seda","family":"Keskin","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Van Yuzuncu Yil University  Van Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,21]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12094-023-03087-8"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/09513590.2020.1753033"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.22271\/j.ento.2021.v9.i3d.8701"},{"issue":"3","key":"e_1_2_11_5_1","first-page":"168","article-title":"Effect of CAPE on Purin Catabolising Enzyme Activities in Bleomycine\u2010Induced Lung Fibrosis","volume":"12","author":"\u00d6zyurt H.","year":"2007","journal-title":"Firat Medical Journal"},{"issue":"1","key":"e_1_2_11_6_1","first-page":"41","article-title":"The Effect of CoQ10 on the Lipid Peroxidation, Antioxidant Profile and Some Biochemical Parameters in Rats Induced With Bleomycin","volume":"20","author":"\u00c7elikezen F. \u00c7.","year":"2009","journal-title":"Van Veterinary Journal"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2006.07474.x"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2018.04.065"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.112503"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1479-828X.2004.00143.x"},{"issue":"1","key":"e_1_2_11_11_1","first-page":"231","article-title":"Lipoic Acid: An Universal Antioxidant","volume":"8","author":"Karaca E. 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