{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:18:18Z","timestamp":1787163498098,"version":"build-2736575974"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T00:00:00Z","timestamp":1746748800000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Rheumatoid arthritis (RA) is a systemic autoimmune disease. Circular RNA_0001715 (circ_0001715) has been demonstrated to be involved in the progression of cancers. This study aimed to discuss the function of circ_0001715 on the development of RA. Tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) was used to active human RA fibroblast\u2010like synoviocytes (FLS) (HFLS\u2010RA) cells. The role of circ_0001715 in the progression of RA was determined by cell counting kit\u20108, flow cytometry, enzyme\u2010linked immunosorbent assay. The target of circ_0001715 was predicted using the circinteractome database and validated by the luciferase reporter assay. The relative protein expression of toll\u2010like receptor (TLR)\u20104\/nuclear factor\u2010kappa B (NF\u2010\u03baB) axis was detected by western blot. Moreover, a collagen\u2010induced arthritis (CIA) mouse model was constructed through the secondary immunization. The role of circ_0001715 in vivo was determined by hematoxylin and eosin (H&amp;E), Safranin O, TRAP, ELISA and western blot. Increased levels of circ_0001715 were discovered in tissues from RA patients, TNF\u2010\u03b1\u2010induced HFLS\u2010RA cells, and synovial tissues of CIA\u2010induced mice. Knockdown of circ_0001715 decreased proliferation and inflammation, but promoted apoptosis of RA both in vitro and in vivo. Additionally, miR\u2010326 was predicted as the target of circ_0001715, which was confirmed by the luciferase reporter assay. Knockdown of miR\u2010326 reversed the results of proliferation, apoptosis and inflammation resulted from the circ_0001715 knockdown. Mechanically, knockdown of circ_0001715 reduced the expression of TLR\u20104\/NF\u2010\u03baB axis, which were rescued by the downregulation of miR\u2010326. Circ_0001715 sequestered miR\u2010326 to regulate the growth, apoptosis and inflammation of HFLS\u2010RA cells via TLR\u20104\/NF\u2010\u03baB axis.<\/jats:p>","DOI":"10.1002\/jbt.70283","type":"journal-article","created":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T05:49:19Z","timestamp":1746769759000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Circ_0001715 Mediated Progression and Inflammation in Fibroblast\u2010Like Synoviocytes of Rheumatoid Arthritis by Targeting miR\u2010326\/TLR\u20104\u2010NF\u2010\u03baB Pathway"],"prefix":"10.1002","volume":"39","author":[{"given":"Min","family":"Niu","sequence":"first","affiliation":[{"name":"Rheumatology Immunology and Endocrinology Department, Honghui Hospital Xi'an Jiaotong University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Geriatric Xi'an Qinhuang Hospital Xi'an Shaanxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Xu","sequence":"additional","affiliation":[{"name":"Rheumatology Immunology and Endocrinology Department, Honghui Hospital Xi'an Jiaotong University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingman","family":"Yuan","sequence":"additional","affiliation":[{"name":"Rheumatology Immunology and Endocrinology Department, Honghui Hospital Xi'an Jiaotong University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meixi","family":"Yan","sequence":"additional","affiliation":[{"name":"Rheumatology Immunology and Endocrinology Department, Honghui Hospital Xi'an Jiaotong University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge","family":"Yang","sequence":"additional","affiliation":[{"name":"Rheumatology Immunology and Endocrinology Department, Honghui Hospital Xi'an Jiaotong University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyi","family":"Yan","sequence":"additional","affiliation":[{"name":"Rheumatology Immunology and Endocrinology Department, Honghui Hospital Xi'an Jiaotong University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9710-429X","authenticated-orcid":false,"given":"Xichao","family":"Yang","sequence":"additional","affiliation":[{"name":"Rheumatology Immunology and Endocrinology Department, Honghui Hospital Xi'an Jiaotong University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.02.001"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2018.1"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2013-204627"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9040880"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.686155"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2017-211741"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kel065"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-020-1135-7"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-016-0055-4"},{"key":"e_1_2_9_11_1","first-page":"269","article-title":"Circles in the Heart and Cardiovascular System","volume":"116","author":"Lim T. 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