{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T20:42:24Z","timestamp":1788295344310,"version":"build-2803163510"},"reference-count":67,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T00:00:00Z","timestamp":1747267200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Experiencing early\u2010life stress (ELS) plays a fundamental role in the development of mood disorders like depression in adulthood. Neuro\u2010immune response in the brain is coupled with depressive\u2010like behaviors following maternal separation (MS) stress. Indomethacin (IND) is a nonsteroidal anti\u2010inflammatory drug. This study aimed to assess the antidepressant\u2010like effect of IND in a mouse model of MS stress, focusing on its potential effect on neuro\u2010inflammatory response in the hippocampus. To do this, male NMRI mice were treated with IND at doses of 5, 10, and 20\u2009mg\/kg, saline (10\u2009mL\/kg) or fluoxetine (30\u2009mg\/kg). Drugs were injected intraperitoneally for 7\u2009days. Behavioral tests including forced swimming test, splash test, and open field test were performed. Quantitative reverse transcription\u2010PCR was used for evaluation of gene expression of inflammatory mediators including of <jats:italic>iNOS<\/jats:italic>, <jats:italic>TLR4<\/jats:italic>, <jats:italic>NLRP3<\/jats:italic>, <jats:italic>TNF\u2010\u03b1<\/jats:italic>, and <jats:italic>IL\u20101\u03b2<\/jats:italic> in the hippocampus. Results showed that MS provoked depressive\u2010like behavior in mice as observed by a decrease in the grooming activity time in the splash test and an increase in the immobility time in the forced swimming test. Depressive\u2010like behaviors were coupled with neuroinflammation in the hippocampus. IND attenuated depressive\u2010like behaviors as well as decreased the gene expression of neuroinflammation\u2010related mediators in the hippocampus of MS mice. In conclusion, results showed that IND, at least in part, via alleviation of neuro\u2010inflammatory response in the hippocampus, exerted antidepressant\u2010like effects in a mouse model of MS stress.<\/jats:p>","DOI":"10.1002\/jbt.70311","type":"journal-article","created":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T05:16:50Z","timestamp":1747286210000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Indomethacin Partially Alleviates Depressive\u2010Like Behaviors in Maternally Separated Mice: Targeting Neuroinflammatory Response in the Hippocampus"],"prefix":"10.1002","volume":"39","author":[{"given":"Milad Nemati","family":"Khoei","sequence":"first","affiliation":[{"name":"Student Research Committee Shahrekord University of Medical Sciences Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narges","family":"Alinaghipour","sequence":"additional","affiliation":[{"name":"Student Research Committee Shahrekord University of Medical Sciences Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6116-4266","authenticated-orcid":false,"given":"Elham","family":"Saghaei","sequence":"additional","affiliation":[{"name":"Medical Plants Research Center Basic Health Sciences Institute Shahrekord University of Medical Sciences Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebrahim","family":"Saedi\u2010Dezaki","sequence":"additional","affiliation":[{"name":"Medical Plants Research Center Basic Health Sciences Institute Shahrekord University of Medical Sciences Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3210-2338","authenticated-orcid":false,"given":"Hossein","family":"Amini\u2010Khoei","sequence":"additional","affiliation":[{"name":"Medical Plants Research Center Basic Health Sciences Institute Shahrekord University of Medical Sciences Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,15]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-21243-x"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12888-018-1625-y"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpsychires.2017.08.020"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.14720"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2005.07.042"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2024.114530"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/brb3.3604"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jdn.10310"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/bcmd.1998.0201"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-014-1762-5"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18010102"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.371344"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/861231"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psyneuen.2012.08.013"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/10520295.2019.1637021"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jad.2010.09.005"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12030753"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0153289"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-9-75"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2019.06.012"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/08923973.2021.1981374"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-9-121"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.909981"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2020.00444"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e15550"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0292631"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.08.060"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2474-11-276"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/13880209.2019.1702704"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-8994-2_9"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00176837"},{"issue":"97","key":"e_1_2_11_33_1","article-title":"The Forced Swim Test as a Model of Depressive\u2010Like Behavior","volume":"2","author":"Yankelevitch\u2010Yahav R.","year":"2015","journal-title":"Journal of Visualized Experiments: JoVE"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnbeh.2022.838122"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.8360"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2013.05.012"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2017.05.015"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-014-1322-2"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2004.11.024"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/brb3.3604"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2202-11-123"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00221-020-05859-y"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2016.04.052"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2006.05.003"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-3057(02)00756-6"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0195521"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2009.11.006"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2018.11.037"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.825522"},{"key":"e_1_2_11_51_1","doi-asserted-by":"crossref","unstructured":"A.Mesripour S.Shahnooshi andV.Hajhashemi \u201cCelecoxib Ibuprofen and Indomethacin Alleviate Depression\u2010Like Behavior Induced by Interferon\u2010Alfa in Mice \u201d 17 no. 1 (2020).","DOI":"10.1515\/jcim-2019-0016"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9020468"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1461145709009924"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibneur.2022.09.001"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2020.112720"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00205.x"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.2165\/00002018-200124110-00002"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/7854_2016_19"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/tpj.2009.68"},{"issue":"3","key":"e_1_2_11_60_1","first-page":"133","article-title":"The NSAIDs Ibuprofen and Celecoxib and the TNF\u2010\u03b1 Blocker Etanercept Prevented Cyclosporine A\u2010Induced Depression\u2010Like Behavior in Mice","volume":"41","author":"Mesripour A.","year":"2021","journal-title":"Hacettepe University Journal of the Faculty of Pharmacy"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12504"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25225388"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.10391"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-5-17"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2005.03480.x"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2011.08.040"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2023.109769"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41398-022-02297-y"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.70311","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T19:45:33Z","timestamp":1758224733000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.70311"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":67,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1002\/jbt.70311"],"URL":"https:\/\/doi.org\/10.1002\/jbt.70311","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"2025-04-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70311"}}