{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T18:37:41Z","timestamp":1780079861741,"version":"3.54.0"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study aimed to investigate the hepatotoxicity of triptolide (TRI) and its mechanism of action. The TRI\u2010liver injury\u2010pyroptosis association and the possible action targets were analyzed through network pharmacology, the molecular\u2010protein docking analysis was conducted by the molecular docking method, and the binding mode between TRI and candidate targets was analyzed with dynamics simulation. In addition, the mouse model of TRI\u2010induced liver injury was constructed in vitro to examine the influence of TRI on liver function. The expression levels of inflammatory factors were detected by ELISA, while pathological analysis was conducted by H&amp;E staining and histochemical staining. As figured out from the network pharmacology analysis results, Caspase\u20103 might be the major action target of TRI, which was verified by molecular docking and dynamics simulation. Besides, the in vitro experimental results demonstrated that TRI induced liver injury in mice, enhanced the tissue inflammatory response. Caspase\u20103 may be the major target of TRI in liver injury, and TRI can mediate pyroptosis and tissue inflammatory response through Caspase\u20103 to induce acute liver injury.<\/jats:p>","DOI":"10.1002\/jbt.70319","type":"journal-article","created":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T07:19:31Z","timestamp":1748330371000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Toxicological Mechanism of Triptolide\u2010Induced Liver Injury"],"prefix":"10.1002","volume":"39","author":[{"given":"Sirui","family":"Hang","sequence":"first","affiliation":[{"name":"Zhejiang Chinese Medical University Hangzhou China"},{"name":"Affiliated Hospital of Jiaxing University Jiaxing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Xu","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Jiaxing University Jiaxing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Wang","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Jiaxing University Jiaxing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0281-3657","authenticated-orcid":false,"given":"Caiqun","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Jiaxing University Jiaxing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenxi","family":"Cao","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Jiaxing University Jiaxing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,27]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15472"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21762"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.119.315861"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.09.038"},{"issue":"16","key":"e_1_2_12_6_1","first-page":"3399","article-title":"Overview of Hepatotoxicity Studies on Tripterygium Wilfordii in Recent 20 Years","volume":"44","author":"Tian Y. 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