{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T20:06:28Z","timestamp":1789589188524,"version":"build-2803163510"},"reference-count":63,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T00:00:00Z","timestamp":1750896000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T00:00:00Z","timestamp":1750896000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>In this study, we evaluated the impact of a potent DNA methyl transferase (DNMT) inhibitor zebularine on imiquimod\u2010induced psoriatic mice, revealing its efficacy in mitigating hyperproliferation and keratinocyte differentiation. Molecular analyses demonstrated a marked inhibition of p38, ERK, and JNK signaling pathways, accompanied by a notable reduction in proliferative markers such as Ki\u201067. The crucial regulator of keratinocyte proliferation, Ki\u201067, exhibited significant downregulation upon zebularine treatment. Moreover, significant anti\u2010inflammatory effects were evident through the suppression of the imiquimod\u2010induced p65 NF\u2010\u03baB signaling cascade. This robust inhibition extended to the reduction in the cytokine storm associated with psoriasis\u2010like skin inflammation. Consequently, there was a restoration of the normal epidermal architecture, accompanied by a decrease in epidermal hyperplasia and splenomegaly. Importantly, zebularine's inhibition of DNMT1 underscores its role in modulating epigenetic pathways. By altering DNA methylation, zebularine might effectively inhibit the expression of genes responsible for both inflammation and proliferation, contributing to improved skin structure. These findings collectively highlight zebularine's potential as a therapeutic agent in the management of psoriasis, supported by its anti\u2010inflammatory effects and observed inhibition of DNMT1.<\/jats:p>","DOI":"10.1002\/jbt.70364","type":"journal-article","created":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T06:01:44Z","timestamp":1750917704000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Zebularine Ameliorates Imiquimod\u2010Induced Psoriasis by Inhibiting Oxidative Stress and Inflammation With Concomitant Inhibition of NF\u2010\u03baB\/MAPK and DNMT1"],"prefix":"10.1002","volume":"39","author":[{"given":"Ankita","family":"Sood","sequence":"first","affiliation":[{"name":"Laboratory of Epigenetics and Diseases, Department of Pharmacology and Toxicology National Institute of Pharmaceutical Education and Research SAS Nagar Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srinidhi","family":"Chintalapani","sequence":"additional","affiliation":[{"name":"Laboratory of Epigenetics and Diseases, Department of Pharmacology and Toxicology National Institute of Pharmaceutical Education and Research SAS Nagar Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivam","family":"Sharma","sequence":"additional","affiliation":[{"name":"Laboratory of Epigenetics and Diseases, Department of Pharmacology and Toxicology National Institute of Pharmaceutical Education and Research SAS Nagar Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3061-9739","authenticated-orcid":false,"given":"Kulbhushan","family":"Tikoo","sequence":"additional","affiliation":[{"name":"Laboratory of Epigenetics and Diseases, Department of Pharmacology and Toxicology National Institute of Pharmaceutical Education and Research SAS Nagar Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,26]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2012.04531.x"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/0378-6323.72443"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1468-3083.2001.00192.x"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00580-015-2061-8"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61128-3"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05663"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.26444\/aaem\/112107"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20184347"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apcsb.2016.09.003"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.111.131912"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12033-009-9216-2"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2011.04261.x"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2016.12.002"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40364-017-0081-z"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2010.07.011"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2012.11.002"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01655-6"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2024.04.012"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/569751"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12895-018-0074-0"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.4103\/bjhs.bjhs_11_17"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.2147\/PTT.S101233"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.2147\/CLEP.S146442"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0802999"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-009-0420-3"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232214045"},{"key":"e_1_2_12_28_1","first-page":"9","article-title":"Formulation, Development and Characterization of Emulgel of a Nsaid's","volume":"1","author":"Kapoor D.","year":"2014","journal-title":"Journal of Clinical Pharmacology"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.5138\/ijdd.2010.0975.0215.02012"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2019.01.006"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.3365"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2015.12.035"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1159\/000373955"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2021.107754"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2013-0195OC"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1080\/13510002.2016.1273437"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2016.10.004"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2022.121873"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-47385-7_2"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.det.2013.04.009"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700340"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2011.05154.x"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20200471"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2019.10.015"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-81947-6_15"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13745"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2017.04.022"},{"issue":"4","key":"e_1_2_12_48_1","first-page":"382","article-title":"Combination of Calcipotriol and Clobetasol Propionate as a Premixed Ointment for the Treatment of Psoriasis","volume":"13","author":"Katoh N.","year":"2003","journal-title":"European Journal of Dermatology: EJD"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.2147\/PTT.S63127"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.4103\/0019-5154.127668"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm13154484"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/561018"},{"key":"e_1_2_12_53_1","unstructured":"M.Yasir A.Goyal andS.Sonthalia Corticosteroid Adverse Effects.2018."},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.4103\/0019-5154.139874"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0320910"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1158\/1940-6207.CAPR-07-0008"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01950"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0091146"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2010.178"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-2212-y"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-022-04523-3"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1089262"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9040857"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.2147\/CCID.S430621"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.70364","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbt.70364","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.70364","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T13:44:09Z","timestamp":1771335849000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.70364"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,26]]},"references-count":63,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1002\/jbt.70364"],"URL":"https:\/\/doi.org\/10.1002\/jbt.70364","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,26]]},"assertion":[{"value":"2025-05-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70364"}}