{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T19:42:41Z","timestamp":1776022961675,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study aimed to demonstrate the protective effect of 1,8\u2010cineole against Aroclor 1254 (A1254)\u2010induced liver toxicity in male rats and to elucidate the underlying mechanisms. A1254 is among the persistent organic pollutants and is a toxic substance that can cause serious damage to various organs such as the liver, brain, and lungs. 1,8\u2010cineole, a monoterpene, possesses significant pharmacological activities such as antioxidant, anti\u2010inflammatory, and anticancer effects. Thirty\u2010two healthy young (4\u22126 weeks) male Wistar albino rats (200\u2013300\u2009g) were used. The animals were randomly divided into four equal groups: Control, Aroclor, Cineole, and Aroclor+Cineole (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20098). Rats were daily administered A1254 (1\u2009mg\/kg, intraperitoneally) either alone or in combination with 1,8\u2010cineole (100\u2009mg\/kg orally in corn oil) for 30 days. The liver tissues and serum were collected from the rats under anesthesia. The protective effect of 1,8\u2010cineole against A1254\u2010induced liver damage was demonstrated using ELISA, RT\u2010PCR, histopathological analysis, and immunohistochemical evaluation methods. A1254 administration increased the total oxidant status (TOS) level and triggered oxidative stress by decreasing the total antioxidant status (TAS). However, 1,8\u2010cineole significantly reduced TOS levels and increased TAS levels in serum and liver tissues (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). A1254 increased the pro\u2010apoptotic Bax gene expression in liver tissues, while 1,8\u2010cineole significantly decreased (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Similarly, A1254 increased the gene expressions of pro\u2010inflammatory cytokines tumor necrosis factor alpha (TNF\u2010\u03b1), interleukin\u20101\u2010beta (IL\u20101\u03b2), inducible nitric oxide synthase (iNOS), and interferon\u2010gamma (INF\u2010\u03b3) in liver tissues, whereas 1,8\u2010cineole decreased the expression of these genes (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Moreover, 1,8\u2010cineole reduced the histopathological changes associated with A1254\u2010induced oxidative stress and inflammation in liver tissues. In conclusion, 1,8\u2010cineole may be a protective agent due to its anti\u2010apoptotic effect, reduction of oxidative damage, anti\u2010inflammatory efficacy, and amelioration of histopathological changes in liver toxicity.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70419","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T12:56:00Z","timestamp":1753707360000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["1,8\u2010Cineole Protects Against Liver Toxicity Induced by Aroclor\u20101254 in a Rat Model"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0433-5125","authenticated-orcid":false,"given":"Hadiqa","family":"Arshad","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine Pamukkale University Kinikli Denizli Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4628-2771","authenticated-orcid":false,"given":"Muhammed Fatih","family":"Do\u011fan","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine Pamukkale University Kinikli Denizli Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00fcnevver Nazl\u0131can","family":"Zengin","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine Pamukkale University Kinikli Denizli Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1835-1082","authenticated-orcid":false,"given":"\u00d6zlem","family":"\u00d6zmen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology, Faculty of Veterinary Medicine Mehmet Akif Ersoy University Burdur Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osman","family":"\u00c7ift\u00e7i","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine Pamukkale University Kinikli Denizli 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