{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T03:41:42Z","timestamp":1792813302003,"version":"4.5.0"},"reference-count":64,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T00:00:00Z","timestamp":1754956800000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Osteoporosis is a condition in where bones gradually become thin and weak, making them more susceptible to fractures. Columbianadin has showed potential anti\u2010inflammatory and antioxidant effect. In this experimental study, anti\u2010osteoporosis effect of Columbianadin against glucocorticoid induced osteoporosis in rats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this study, Sprague Dawley (SD) rats were used and osteoporosis was induced by administering dexamethasone (DEX) at a dosage of 2.5\u2009mg\/kg\/day. Following induction, the rats were treated orally with different doses of columbianadin. The body weight, organ (femoral, vagina and uterus) weights were measured. The bone parameters, antioxidant, cytokines, hormones were evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Columbianadin improved body weight and altered the organ weight such as femoral, vagina and uterus. It also modulated the various bone related parameters including bone mineral density (BMD), tissue mineral density (TMD), bone mineral content (BMC), tissue mineral content (TMC); bone parameters like tartrate\u2010resistant acid phosphatase (TRAP), \u03b2 \u2010C\u2010terminal telopeptide of type I collagen (\u03b2\u2010CTX), osteocalcin (OC), bone gla protein (BGP), acid phosphatase (ACP), alkaline phosphatase (ALP). Additionally, Columbianadin influence hormone level such as estradiol (E\n                      <jats:sub>2<\/jats:sub>\n                      ) and parathyroid hormone (PTH) and enhanced antioxidant defense via modulating glutathione peroxidase (GPx), thiobarbituric acid reactive substances (TBARS), catalase (CAT), superoxide dismutase (SOD), glutathione (GSH). It also regulated the cytokines parameters like tumor necrosis factor\u2010 \u03b1 (TNF\u2010\u03b1), interleukin\u20101\u03b2 (IL\u20101\u03b2), interleukin\u20106 (IL\u20106), insulin\u2010like growth factor (IGF), transforming growth factor\u2010\u03b2 (TGF\u2010\u03b2). Furthermore, columbianadin modulated the levels of receptor activator of nuclear factor\u2010\u03baB ligand (RANKL), osteoprotegerin (OPG) and OPG\/RANKL ratio. Columbianadin also altered mRNA expression of ALP, OPN, runt\u2010related transcription factor 2 (RunX2), OCN, bone morphogenetic proteins (BMP)\u20102, 4, 6, 7, 9, B\u2010cell lymphoma 2 (BcL\u20102), B\u2010cell lymphoma\u2010extra\u2010large (BcLxL), Inhibitor of Apoptosis Protein (IAP) 1, 2, X\u2010linked Inhibitor of apoptosis Protein (XIAP), caspase\u20103,6,7,9, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The finding of the study clearly demonstrates that columbianadin exerts a significant antiosteoporosis effect against glucocorticoid\u2010induced osteoporosis in rats.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jbt.70432","type":"journal-article","created":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T08:07:09Z","timestamp":1754986029000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Columbianadin Ameliorate Osteoporosis Against Glucocorticoid Induced Osteoporosis in Rats via Alteration of RANK\/RANKL\/OPG Signaling Pathway"],"prefix":"10.1002","volume":"39","author":[{"given":"Songbo","family":"Mao","sequence":"first","affiliation":[{"name":"Orthepadic Department The 960th Hospital of the PLA Joint Logistics Support Force Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuntao","family":"Xu","sequence":"additional","affiliation":[{"name":"Orthepadic Department The 960th Hospital of the PLA Joint Logistics Support Force Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-0401-161X","authenticated-orcid":false,"given":"Lin","family":"Zou","sequence":"additional","affiliation":[{"name":"Orthepadic Department The 960th Hospital of the PLA Joint Logistics Support Force Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,12]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"18","article-title":"Protective Role of \u03b2\u2010Sitosterol in Glucocorticoid\u2010Induced Osteoporosis in Rats via the RANKL\/OPG Pathway","volume":"28","author":"Wang T.","year":"2022","journal-title":"Alternative Therapies in Health and Medicine"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.932488"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S225516"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110747"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43450-023-00383-w"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23010377"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3803\/ENM.2021.1021"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.2217\/17455057.2.1.65"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.2217\/ijr.14.24"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.4163\/jnh.2019.52.4.323"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1055\/a-2112-1596"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11040506"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12212576"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2015.03.005"},{"key":"e_1_2_11_16_1","doi-asserted-by":"crossref","unstructured":"E.Gaffney\u2010Stomberg M. 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