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The rats were distributed into five groups. The first group served as a control, the second group received MitoQ (2\u2009mg\/kg\/day; i.p.) for 7 days, the third group was exposed to gamma radiation (5 gray as a single dose), the fourth group received MitoQ prophylactically before irradiation, and the last group was exposed to gamma radiation and then treated with MitoQ. The rats were killed 7 days after irradiation. MitoQ replenished mitochondrial ROS, indicating its antioxidant effect. MitoQ inhibited the intrinsic apoptosis cascade via a reduction in Bax and alleviated Bcl\u20102. MitoQ improved steroidogenesis, as verified by an increase in testosterone and upregulation of 3\u03b2\u2010HSD and 17\u03b2\u2010HSD expression, in addition to increasing complex I and succinate dehydrogenase activity. Likewise, sperm evaluation corroborated these findings. The acute toxicity profile of MitoQ was investigated in mice and was found to be 75\u2009mg\/kg body weight. Additionally, a molecular docking study of MitoQ showed a good fit to the active site of succinate dehydrogenase. In conclusion, this study introduces MitoQ as a new approach for the management of testicular damage triggered by gamma irradiation.<\/jats:p>","DOI":"10.1002\/jbt.70447","type":"journal-article","created":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T05:40:01Z","timestamp":1755063601000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["MitoQ Mitigates Radiation\u2010Induced Testicular Injury: Molecular Docking and Toxicity Implications"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1862-405X","authenticated-orcid":false,"given":"Ayman A.","family":"Ibrahim","sequence":"first","affiliation":[{"name":"Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT) Egyptian Atomic Energy Authority (EAEA) Cairo Egypt"},{"name":"Department of Chemistry and Chemical Biology McMaster University Hamilton ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4788-5016","authenticated-orcid":false,"given":"Marwa G.","family":"El\u2010Gazzar","sequence":"additional","affiliation":[{"name":"Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT) Egyptian Atomic Energy Authority (EAEA) Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5929-6932","authenticated-orcid":false,"given":"Heba M.","family":"Karam","sequence":"additional","affiliation":[{"name":"Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT) Egyptian Atomic Energy Authority (EAEA) Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfj178"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/HP.0000000000001601"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/09553002.2012.717732"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-031-18810-7_1"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines12071492"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(98)00167-1"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/CM9.0000000000003478"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553002.2017.1355579"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2013.07.024"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2010.05627.x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmp016"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2018.03.024"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1667\/RR1737.1"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204054"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/8575263"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00026.2013"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M009093200"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22094753"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.861622"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2023.122063"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00067.2023"},{"key":"e_1_2_10_23_1","unstructured":"K.Dawes J.Melnik M.Lyons et al. 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