{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:16:23Z","timestamp":1791072983809,"version":"4.1.0"},"reference-count":69,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T00:00:00Z","timestamp":1756857600000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The objective of this study was to examine the chemotherapeutic effect of CAPE, via the mitochondrial membrane potential (MMP, \u0394\u03c8m) pathway in TPC\u20101 human papillary thyroid cancer cells. The cytotoxic effect of CAPE was evaluated using MTT and crystal violet assays, while its apoptotic activity was measured using Bax, Bcl\u20102, Caspase\u20103,\u20108,\u20109 and Apaf\u20101 assays. Effects on mitochondria were performed by analyzing JC\u20101 fluorescent probe\u2010MMP, ROMO1 and mitochondrial ATP\u2010synthase. The analysis of ROS and 8\u2010OHdG was undertaken to assess the degree of oxidative stress and DNA damage, while LDH analysis was used as a marker of both cytotoxicity and cellular membrane damage. To determine antimetastatic activity, cell migration and colony formation assays were performed. Finally, Giemsa staining was chosen for cytomorphological analysis. CAPE treatment in TPC\u20101 cells was selected as the effective dose (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 25\u2009\u00b5M\/48\u2009h) for further experiments, and it was found that this reduced Bcl\u20102 levels and increased the activation of key components Bax, Caspase\u20103,\u20108,\u20109 and Apaf\u20101, indicating that CAPE\u2010induced cell death was apoptosis\u2010dependent. The study revealed that CAPE induced mitochondrial depolarization, leading to a substantial decrease in mitochondrial ATP\u2010synthase, along with a notable increase in intracellular ROS, ROMO1 levels, and 8\u2010OHdG DNA damage and extracellular LDH. Furthermore, CAPE exhibited a significant inhibitory effect on cell migration and colony formation, accompanied by cytomorphological changes. In conclusion, this study demonstrates that CAPE, which shows bioactivity by modulating MMP, can target several hallmarks of cancer in TPC\u20101 cells and therefore has an important potential for future in vivo research.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70487","type":"journal-article","created":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T16:28:01Z","timestamp":1756916881000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Therapeutic Potential of CAPE in Targeting Hallmarks of Cancer in TPC\u20101 Thyroid Cancer Cells Through Modulation of Mitochondrial Membrane Potential"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7312-5934","authenticated-orcid":false,"given":"Yasin","family":"T\u00fcl\u00fcce","sequence":"first","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine Van Y\u00fcz\u00fcnc\u00fc Y\u0131l University Van T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8273-9800","authenticated-orcid":false,"given":"H\u00fcmeyra","family":"Bucak","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Institute of Health Sciences Van Y\u00fcz\u00fcnc\u00fc Y\u0131l University Van T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3617-0023","authenticated-orcid":false,"given":"Sedat","family":"K\u00f6stekci","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Institute of Natural and Applied Sciences Van Y\u00fcz\u00fcnc\u00fc Y\u0131l University Van T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,3]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"\u201cThyroid Cancer Incidence and Mortality Females in 2022 \u201d WHO published 2024 https:\/\/gco.iarc.fr\/today\/en\/dataviz\/bars?types=0_1&mode=cancer&group_populations=1&sort_by=value1&sexes=2&populations=792."},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(23)00020-X"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.82.11.4386"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.86.4.7390"},{"key":"e_1_2_11_6_1","first-page":"12","article-title":"Lung Metastases From Differentiated Thyroid Carcinoma. 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