{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T05:45:22Z","timestamp":1782366322998,"version":"3.54.5"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study investigates the role of allograft inflammatory factor 1 (AIF\u20101) in regulating the proliferation, invasion, and migration of endometrial stromal cells (ESCs) in the context of endometriosis, focusing on its impact on mitochondrial function. Ectopic endometrial tissues were collected from patients diagnosed with endometriosis, and normal endometrial tissues served as controls. ESCs were isolated and cultured. AIF\u20101 expression was knocked down using siRNA and overexpressed using plasmid vectors. Quantitative real\u2010time PCR (qRT\u2010PCR), western blot analysis, CCK\u20108 assays, flow cytometry, transwell assays, scratch assays, ATP detection, and mitochondrial membrane potential assays were performed to evaluate gene expression, cell proliferation, apoptosis, invasion, and mitochondrial function. AIF\u20101 mRNA and protein levels were significantly upregulated in ectopic ESCs compared to controls. Overexpressing AIF\u20101 elevated cell proliferation and invasion and decreased apoptosis. Additionally, AIF\u20101 knockdown decreased mitochondrial DNA copy number, membrane potential, and ATP levels, whereas its overexpression had the opposite effects. AIF\u20101 plays a crucial role in ESCs proliferation, invasion, and migration by modulating mitochondrial function, potentially via the AIF\u20101 pathway. These findings suggest that targeting AIF\u20101 could be a novel therapeutic approach for managing endometriosis.<\/jats:p>","DOI":"10.1002\/jbt.70493","type":"journal-article","created":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T14:44:36Z","timestamp":1758206676000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["AIF\u20101 Modulates Endometrial Stromal Cell Proliferation, Invasion, and Migration via Mitochondrial Function"],"prefix":"10.1002","volume":"39","author":[{"given":"Bo","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Gynecology and Obstetrics, Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, Shanghai Tenth People's Hospital Tongji University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3875-210X","authenticated-orcid":false,"given":"Qigui","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, Shanghai Tenth People's Hospital Tongji University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuni","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, Shanghai Tenth People's Hospital Tongji University School of Medicine Shanghai China"},{"name":"Tongji University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,18]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/17482631.2020.1822621"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.23736\/S0026-4784.17.04048-5"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2008.04.056"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-38692-8"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12416"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600461"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2007.01.001"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2009.12.008"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2016.02.011"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2016.05.004"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dey119"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2004-0871"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2019.12.002"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43032-025-01827-5"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43032-021-00831-9"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxh316"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics11081434"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dez266"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-011-1133-7"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80299-3"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-014-9515-3"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms221910498"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40824-023-00442-2"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3NR03527A"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph17030311"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2025.5513"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/4809415"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.204631"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.70493","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbt.70493","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.70493","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T05:58:23Z","timestamp":1775973503000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.70493"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":28,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1002\/jbt.70493"],"URL":"https:\/\/doi.org\/10.1002\/jbt.70493","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"2024-10-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70493"}}