{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T11:23:41Z","timestamp":1784633021187,"version":"3.55.0"},"reference-count":53,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T00:00:00Z","timestamp":1757894400000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The work aimed at exploring the potential mechanism of Long noncoding RNA tyrosine protein kinase transmembrane receptor 1 antisense RNA 1 (ROR1\u2010AS1) in the process of epithelial\u2010mesenchymal transition (EMT) of pancreatic cancer (PC) cells resistant to Gemcitabine (GEM). ROR1\u2010AS1 expression was analyzed in PC tissues, cells, PANC\u20101\/GEM\u2010resistant cell lines, and its correlation with PC clinicopathological features was determined. The proliferation ability, invasive and migratory properties of PC cells were evaluated, and the apoptotic rate was measured. EMT process of E\u2010cadherin, N\u2010cadherin, and Snail were analyzed. The direct molecular interactions within the ROR1\u2010AS1\/miR\u201033a\u20105p\/HMGB1 axis were confirmed using dual\u2010luciferase reporter and RNA immunoprecipitation assays. ROR1\u2010AS1 expression was elevated in PC tissues, cells, and PANC\u20101\/GEM cells. The survival rate of patients with higher ROR1\u2010AS1 levels was lower, and expression was correlated with tumor node metastasis stage and lymph node metastasis. Silencing ROR1\u2010AS1 supressed cell growth and EMT process, whilst overexpressing ROR1\u2010AS1 was the opposite. ROR1\u2010AS1 could bind miR\u201033a\u20105p to target and regulate HMGB1 and affect the biological behaviors of cancer cells. The suppressive influence of ROR1\u2010AS1 knockdown was rescued when miR\u201033a\u20105p was silenced, meanwhile, the promoting influence of ROR1\u2010AS1 overexpression was mitigated by silencing HMGB1. Cells resistant to GEM became more sensitive to GEM when miR\u201033a\u20105p was overexpressed or ROR1\u2010AS1 was silenced, whereas the improvement of GEM resistance was offset by inhibiting miR\u201033a\u20105p or upregulating HMGB1. ROR1\u2010AS1 modulates the growth and EMT process of PC through the miR\u201033a\u20105p\/HMGB1 axis. ROR1\u2010AS1 knockdown or miR\u201033a\u20105p overexpression is effective to reduce GEM resistance in PC.<\/jats:p>","DOI":"10.1002\/jbt.70499","type":"journal-article","created":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T15:52:04Z","timestamp":1757951524000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["LncRNA ROR1\u2010AS1 Stimulates Epithelial\u2010Mesenchymal Transition and Chemoresistance of Pancreatic Cancer by Upregulating HMGB1 Through miR\u201033a\u20105p Adsorption"],"prefix":"10.1002","volume":"39","author":[{"given":"RenZhe","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Oncology, First Affiliated Hospital Heilongjiang University of Chinese Medicine Harbin City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8245-8263","authenticated-orcid":false,"given":"XiaoQing","family":"You","sequence":"additional","affiliation":[{"name":"Heilongjiang University of Chinese Medicine Harbin City China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,15]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-020-0363-5"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21551"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2021.13027"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00331.2022"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v29.i3.579"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2011.384"},{"issue":"85","key":"e_1_2_12_8_1","first-page":"367","article-title":"Novel Targeted Therapies in Peripheral T Cell Lymphoma","volume":"15","author":"Jagadeesh D.","year":"2013","journal-title":"Discovery Medicine"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.2013.816282"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-10-38"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmedgenet-2015-103334"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2521"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26537"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.10891"},{"issue":"12","key":"e_1_2_12_15_1","first-page":"5496","article-title":"The lncRNA\u2010HOXA\u2010AS2\/EZH2\/LSD1 Oncogene Complex Promotes Cell Proliferation in Pancreatic Cancer","volume":"9","author":"Lian Y.","year":"2017","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384047.2017.1385682"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.10545"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2012.193"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2021.8010"},{"issue":"4","key":"e_1_2_12_20_1","first-page":"1764","article-title":"Long Non\u2010Coding RNA ROR1\u2010AS1 Enhances Colorectal Cancer Metastasis by Targeting miR\u2010375","volume":"25","author":"Wang F. 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