{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T20:17:51Z","timestamp":1788293871812,"version":"build-2803163510"},"reference-count":53,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T00:00:00Z","timestamp":1758412800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Diminished ovarian reserve (DOR) is a multifactorial gynecological disorder that has emerged as a significant global health challenge. Currently, there are no effective preventive or therapeutic strategies for DOR. Exosome\u2010derived long non\u2010coding RNAs (lncRNA) in follicular fluid (FF) plays a crucial role in follicular development. We identified exosome\u2010derived lncRNA LIPE\u2010AS1 from the FF of DOR patients, which regulates histone deacetylase 3 (HDAC3) expression by competitively binding to miR\u2010330\u20105p. Exosomes, as nanosized membrane vesicles, can deliver therapeutic agents in a targeted manner through ligand modification. In this study, we employed engineered exosomes combined with lncRNA for ovary\u2010targeted therapy of DOR. First, we elucidated the role of lncRNA LIPE\u2010AS1 in the pathogenesis of DOR. Next, we generated exosomes with high LIPE\u2010AS1 expression (Exo\u2010LIPE\u2010AS1) using 293\u2009T cells. Co\u2010culture of Exo\u2010LIPE\u2010AS1 with oocytes from DOR models enhanced oocyte maturation and improve oocyte quality in vitro. Finally, we developed FSH\u03b2\u2010modified, LIPE\u2010AS1\u2010loaded exosomes (Exo<jats:sub>FSH\u03b2<\/jats:sub>\u2010LIPE\u2010AS1), which demonstrated enhanced ovarian delivery efficiency in vivo. Consequently, Exo<jats:sub>FSH\u03b2<\/jats:sub>\u2010LIPE\u2010AS1improved fertility outcomes in DOR models. Our findings demonstrate that exosomes serve as effective targeted vehicles for lncRNA LIPE\u2010AS1, offering potential preventive and therapeutic benefits for DOR.<\/jats:p>","DOI":"10.1002\/jbt.70519","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T03:00:15Z","timestamp":1758510015000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Exosome\u2010Derived lncRNA LIPE\u2010AS1 Enhances Oocytes Maturation and Ameliorates Diminished Ovarian Reserve via the miR\u2010330\u20105p\/HDAC3 Axis"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8594-9519","authenticated-orcid":false,"given":"Jialing","family":"Li","sequence":"first","affiliation":[{"name":"Reproductive Medicine Center General Hospital of Ningxia Medical University Yinchuan Ningxia China"},{"name":"Ningxia Key Laboratory of Clinical and Pathogenic Microbiology General Hospital of 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