{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T02:19:51Z","timestamp":1777515591160,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This study aimed to investigate the effects of oleuropein (Ole) on prostate cancer (PCa) cells and its underlying mechanisms, particularly whether it mediates DNA damage through HIF\u20101\u03b1. PCa cells DU145 were treated with different concentrations of Ole (25, 50, 100, 250, and 500\u2009\u03bcM) or transfected with HIF\u20101\u03b1 overexpression plasmid. The 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide (MTT) assay was used to detect the cell viability; flow cytometry was used to detect apoptosis; quantitative reverse transcription polymerase chain reaction and western blot were employed to measure HIF\u20101\u03b1 expression levels. Additionally, comet assay and western blot were employed to evaluate DNA damage and the expression of DNA damage\u2010related proteins (\u03b3H2AX and p53). Ole significantly inhibited DU145 cell viability in a concentration\u2010dependent manner (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). Additionally, apoptosis rates increased significantly with increasing Ole concentrations (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). Ole induced DNA damage in DU145 cells. Notably, overexpression of HIF\u20101\u03b1 partially reversed the effects of Ole on PCa cells. Ole inhibits DU145 cell viability, promotes apoptosis, and induces DNA damage, possibly through the HIF\u20101\u03b1 pathway. HIF\u20101\u03b1 plays a potential role in the anticancer effects of Ole, providing new experimental evidence for its application in PCa research.<\/jats:p>","DOI":"10.1002\/jbt.70524","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T07:08:15Z","timestamp":1759216095000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Oleuropein Promotes DNA Damage in Prostate Cancer Cells via HIF\u20101\u03b1 Downregulation"],"prefix":"10.1002","volume":"39","author":[{"given":"Hanping","family":"Wei","sequence":"first","affiliation":[{"name":"Department of Urology Wujin Hospital Affiliated With Jiangsu University Changzhou China"},{"name":"Department of Urology The Wujin Clinical College of Xuzhou Medical University Changzhou 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