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Cell counting kit\u20108, transwell, iron measurement, the determination of ROS levels and western blot were utilized to examine the impact and mechanism of KF on melanoma. An in vivo investigation employing immunohistochemistry and western blot assays was carried out on tumor\u2010bearing mice. A375 and A2085 cells' E\u2010cadherin expression was boosted whereas N\u2010cadherin expression, invasion count, and cell survival were all decreased by KF. In both A375 and A2085, KF raised the relative Fe<jats:sup>2+<\/jats:sup> level, which was then farther enhanced by the erastin incubation and reduced by the ferrostatin\u20101 treatment. Besides, KF enhanced the level of ROS while lowering the expression of xCT and GPX4 in the two cells. Mechanically, the application of LiCl reversed the drop in the relative levels of p\u2010GSK3\u03b2\/GSK3\u03b2, c\u2010Myc, and active\u2010\u03b2\u2010catenin\/\u03b2\u2010catenin in melanoma cells caused by KF. LiCl's activation of the Wnt\/\u03b2\u2010catenin pathway counteracted the impact of KF on melanoma cell proliferation, invasion, and ferroptosis. KF reduced tumor weight and volume in vivo, as well as the expression of c\u2010Myc, p\u2010GSK3\u03b2\/GSK3\u03b2, active\u2010\u03b2\u2010catenin\/\u03b2\u2010catenin, and N\u2010cadherin, GPX4, and xCT, but enhanced the E\u2010cadherin level in mice xenografted with A375 cells. KF inhibited melanoma by attenuating proliferation and invasion and inducing ferroptosis via Wnt\/\u03b2\u2010catenin axis, highlighting the underlying therapeutical effect of KF on melanoma.<\/jats:p>","DOI":"10.1002\/jbt.70535","type":"journal-article","created":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T18:04:03Z","timestamp":1759687443000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Kaempferide Inhibits the Proliferation and Invasion and Induces Ferroptosis in Melanoma by Inactivating Wnt\/\u03b2\u2010Catenin Pathway"],"prefix":"10.1002","volume":"39","author":[{"given":"Yonghui","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Endocrinology The People's Hospital of Qiannan Duyun Guizhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Qiannan Medical College for Nationalities Duyun Guizhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeping","family":"Huang","sequence":"additional","affiliation":[{"name":"The Department of Outpatient The Sixth Medical Center of Chinese PLA General Hospital Beijing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Department of Outpatient The Sixth Medical Center of Chinese PLA General Hospital Beijing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2105-5556","authenticated-orcid":false,"given":"Runping","family":"Yang","sequence":"additional","affiliation":[{"name":"The Department of Dermatology The Sixth Medical Center of Chinese PLA General Hospital Beijing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory The People's Hospital of Qiannan Duyun Guizhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.21935"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/medsci9040063"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamadermatol.2022.0160"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)31559-9"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1103782"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)60868-X"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1203421"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2014.56.2736"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1802357"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines10040822"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.03.042"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-nutr-082117-051732"},{"key":"e_1_2_10_14_1","first-page":"1933","article-title":"Iron and Magnetic: New Research Direction of the Ferroptosis\u2010Based Cancer Therapy","volume":"8","author":"Wang S.","year":"2018","journal-title":"American Journal of Cancer Research"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2021.03.022"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/10715762.2019.1666983"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2019.04.002"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-023-01296-w"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-024-03220-9"},{"key":"e_1_2_10_20_1","first-page":"81","article-title":"Antioxidant Capacity and Radioprotective Propertiesof the Flavonoids Galangin and Kaempferide Isolated From Alpinia galanga L. 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