{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T19:00:10Z","timestamp":1785438010153,"version":"3.56.0"},"reference-count":41,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A series of 3\u2010(2\u2010oxo\u20102\u2010arylethylidene)indolin\u20102\u2010ones were synthesized and evaluated for cytotoxic efficacy in the prostate cancer cell lines (PC\u20103 and DU145), and the colon cancer cell line (HCT\u2010116), and in noncancerous Madin\u2010Darby canine kidney (MDCK) cells. The majority\u00a0of the evaluated compounds had cytotoxic efficacy (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009&lt;\u200910\u2009\u00b5M) in the prostate and colon cancer cell lines. Compound 3j (Raji 10), with a 4\u2010Me substitution on the phenyl ring, was the most potent in decreasing the proliferation of prostate cancer cells (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009&lt;\u20095\u2009\u00b5M; 3.56\u2009\u00b1\u20090.49\u2009\u00b5M in PC\u20103 and 4.20\u2009\u00b1\u20090.62\u2009\u00b5M in DU145). Similarly, compound 3o (Raji 16), with a 5\u2010Br substitution in the isatin moiety and 4\u2010F substitution in the phenyl ring, had maximal cytotoxic potency in HCT\u2010116 cells (IC\n                    <jats:sub>50<\/jats:sub>\n                    value of 2.93\u2009\u00b1\u20090.49\u2009\u00b5M). Furthermore, the IC\n                    <jats:sub>50<\/jats:sub>\n                    value of compounds, Raji 10 and Raji 16 was significantly greater (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009&gt;\u200960\u2009\u03bcM) in the MDCK cells, compared to the prostate and colorectal cancer cells (HCT\u2010116). The mechanism of cell death induced by the lead compounds; 3j (Raji 10) and 3o (Raji 16) in PC\u20103 and HCT\u2010116 cells, respectively, was determined. Compounds Raji 10 and Raji 16 induced apoptotic cell death by activating the intrinsic apoptotic pathway, accompanied by an increase in the levels of reactive oxygen species, induction of nuclear fragmentation, and cell cycle arrest at the G1 phase in HCT\u2010116 cells and S\/G2 phase in PC\u20103 cells. Overall, the in vitro results suggest the potential of these compounds in the management of prostate and colon cancer.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70545","type":"journal-article","created":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T06:42:16Z","timestamp":1761115336000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification and Mechanistic Profiling of Indolin\u20102\u2010One Derivatives That Induce ROS\u2010Driven Intrinsic Apoptosis in Prostate and Colorectal Cancer Cells"],"prefix":"10.1002","volume":"39","author":[{"given":"Rabin","family":"Neupane","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Experimental Therapeutics, College of Pharmacy &amp; Pharmaceutical Sciences University of Toledo Toledo Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandrabose","family":"Karthikeyan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy University of Arkansas for Medical Sciences Little Rock Arkansas USA"},{"name":"Department of Pharmacy Indira Gandhi National Tribal University Amarkantak India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1947-0195","authenticated-orcid":false,"given":"Saloni","family":"Malla","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Experimental Therapeutics, College of Pharmacy &amp; Pharmaceutical Sciences University of Toledo Toledo Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joyeeta T.","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy University of Arkansas for Medical Sciences Little Rock Arkansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles R.","family":"Ashby","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy &amp; Pharmaceutical Sciences St. John's University New York City New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narayana","family":"Subbiah Hari Narayana Moorthy","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Indira Gandhi National Tribal University Amarkantak India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piyush","family":"Trivedi","sequence":"additional","affiliation":[{"name":"Center of Innovation and Translational Research, Poona College of Pharmacy Bharati Vidyapeeth Deemed University Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5798-1431","authenticated-orcid":false,"given":"Harlokesh","family":"Yadav","sequence":"additional","affiliation":[{"name":"Department of Pharmacology All India Institute of Medical Sciences Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0337-2291","authenticated-orcid":false,"given":"R.","family":"Jayachandra Babu","sequence":"additional","affiliation":[{"name":"Department of Drug Discovery &amp; Development, Harrison School of Pharmacy Auburn University Auburn Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nageeb","family":"Hassan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences Ajman University Ajman UAE"},{"name":"Centre of Medical and Bio\u2010allied Health Sciences Research Ajman University Ajman UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sai H. 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