{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T13:18:14Z","timestamp":1789564694823,"version":"build-2803163510"},"reference-count":44,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Salvianolic acid B (Sal\u2010B) is a well\u2010recognized bioactive ingredient showing certain effects against ischemia\/reperfusion (I\/R) injury in different organs. This article investigated Sal\u2010B's effects on regulating intestinal bacterial\/endotoxin translocation postintestinal I\/R injury via the TLR4\/MyD88\/MAPK pathway in rats. The rat models of intestinal I\/R injury were established by reperfusion following 60\u2009min of superior mesenteric artery occlusion. Rats were treated with Sal\u2010B after I\/R operation. The histopathological changes of the intestine were assessed by H&amp;E staining, and intestinal tissue injury was evaluated based on the Chiu's score system. Pro\u2010inflammatory factor levels (tumor necrosis factor\u2010\u03b1 [TNF\u2010\u03b1], interleukin [IL]\u20101\u03b2, and IL\u20106), superoxide dismutase (SOD) activity, malondialdehyde (MDA) level, and myeloperoxidase (MPO) activity in intestinal tissues were measured through ELISA and kits. The bacterial\/endotoxin translocation after intestinal I\/R injury was detected by bacterial culture of tissues and Limulus Amebocyte Lysate quantitative kits. The TLR4\/MyD88\/MAPK pathway\u2010associated protein levels were measured by western blot analysis. The TLR4\u2010MyD88 interaction was analyzed via co\u2010immunoprecipitation. Sal\u2010B attenuated intestinal I\/R injury in rats, evidenced by decreased pathological alterations in intestinal tissues, lowered Chiu's histological score, elevated SOD activity, and diminished MDA level, MPO activity, and pro\u2010inflammatory cytokine levels. Moreover, Sal\u2010B reduced intestinal I\/R\u2010triggered intestinal bacterial\/endotoxin translocation. Sal\u2010B could downregulate MyD88 by suppressing TLR4. Inhibition of the TLR4\/MyD88 pathway alleviated intestinal bacterial\/endotoxin translocation, while activation of this pathway partially counteracted the improvement effects of Sal\u2010B in rats with intestinal I\/R injury. Sal\u2010B impeded the MAPK pathway by repressing TLR4\/MyD88. Sal\u2010B mitigates intestinal I\/R\u2010induced intestinal bacterial\/endotoxin translocation in rats by blocking the TLR4\/MyD88\/MAPK pathway.<\/jats:p>","DOI":"10.1002\/jbt.70551","type":"journal-article","created":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T06:55:18Z","timestamp":1761116118000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Salvianolic Acid B Regulates Intestinal Bacterial\/Endotoxin Translocation After Intestinal Ischemia\/Reperfusion Injury in Rats Through TLR4\/MyD88\/MAPK Pathway"],"prefix":"10.1002","volume":"39","author":[{"given":"Rushan","family":"Fei","sequence":"first","affiliation":[{"name":"Department of Colorectal Surgery, The First Affiliated Hospital Zhejiang University School of Medicine Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saisai","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Colorectal Surgery, The First Affiliated Hospital Zhejiang University School of Medicine Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4039-7679","authenticated-orcid":false,"given":"Tao","family":"Shang","sequence":"additional","affiliation":[{"name":"Department of Colorectal Surgery The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine) Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,21]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.750731"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v22.i9.2760"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.111482"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00423-010-0727-x"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/13510002.2016.1229893"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2005.02844.x"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2016.00134"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-019-01755-7"},{"key":"e_1_2_12_10_1","first-page":"30","article-title":"Protective Effect of Salvianolic Acid B on Intestinal Ischemia\u2010Reperfusion Injury in Rats","volume":"42","author":"Chen G. 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