{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:59:38Z","timestamp":1785437978426,"version":"3.56.0"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Stomach cancer remains the leading cause of cancer\u2010related mortality worldwide and necessitates the development of novel therapeutic agents. Marine\u2010derived natural products as promising anticancer agents. In this study, we investigated the potential therapeutic effects of taichunamide\u2010A (TAI\u2010A), a marine fungal metabolite isolated from\n                    <jats:italic>Aspergillus taichungensis 299<\/jats:italic>\n                    , against stomach cancer. We evaluated the anticancer properties of TAI\u2010A in AGS and HGC\u201027 stomach cancer cell lines using cell viability, flow cytometry, and colony formation assays. The molecular mechanisms were investigated using RNA sequencing, pathway analysis, qPCR, and Western blot analysis. We also assessed the potential synergistic effects of TAI\u2010A and the epidermal growth factor receptor (EGFR) inhibitor, erlotinib. TAI\u2010A demonstrated potent antiproliferative activity against stomach cancer cells (EC\n                    <jats:sub>50<\/jats:sub>\n                    : AGS\u2009=\u20090.35\u2009\u03bcM, HGC\u201027\u2009=\u20090.23\u2009\u03bcM) and significantly suppressed colony formation. Mechanistic studies revealed that TAI\u2010A induced apoptosis through simultaneous inhibition of the AKT and MAPK signaling pathways. Transcriptomic analysis revealed the downregulation of cell cycle\u2010related genes and enrichment of apoptotic pathways. Notably, TAI\u2010A exhibited synergistic effects with erlotinib, enhancing antitumor activity by inducing apoptosis. Our findings established TAI\u2010A as a promising anticancer agent that targets multiple oncogenic pathways in stomach cancer. The synergistic interaction with EGFR inhibitors suggested potential therapeutic applications in combination treatment strategies. These results warrant further investigation into TAI\u2010A in preclinical models.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70571","type":"journal-article","created":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T06:48:52Z","timestamp":1761115732000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Taichunamide\u2010A Inhibits Stomach Cancer Growth Through AKT\/MAPK Pathway Suppression and EGFR Inhibitor Synergy"],"prefix":"10.1002","volume":"39","author":[{"given":"Lian\u2010cheng","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Gastrointestinal Surgery 2 Section, Institute of Abdominal Surgery, Key Laboratory of Accurate Diagnosis and Treatment of Cancer The First Affiliated Hospital of Fujian Medical University Fuzhou China"},{"name":"Department of General Surgery Jiangsu Province (Suqian) Hospital Suqian Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010huan","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of General Surgery Jiangsu Province (Suqian) Hospital Suqian Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8159-2778","authenticated-orcid":false,"given":"Shan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of General Surgery Jiangsu Province (Suqian) Hospital Suqian Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo\u2010dong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of General Surgery Jiangsu Province (Suqian) Hospital Suqian Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of General Surgery Jiangsu Province (Suqian) Hospital Suqian Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9512-3700","authenticated-orcid":false,"given":"Jian\u2010Xin","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery 2 Section, Institute of Abdominal Surgery, Key Laboratory of Accurate Diagnosis and Treatment of Cancer The First Affiliated Hospital of Fujian Medical University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,21]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-021-00412-7"},{"issue":"10","key":"e_1_2_12_3_1","first-page":"1378","article-title":"Genetic Risk, Incident Gastric Cancer, and Healthy Lifestyle: A Meta\u2010Analysis of Genome\u2010Wide Association Studies and Prospective Cohort Study","volume":"21","author":"Jin 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