{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T04:54:09Z","timestamp":1787979249057,"version":"build-2784847793"},"reference-count":42,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Paraquat (PQ) is extremely toxic to humans and leads to hepatic inflammation. Fucoidan (FCN) regulates the progression of oxidative damage and inflammation. However, whether FCN mitigates PQ\u2010induced hepatic damage remains undiscovered. This study aimed to elucidate the protective effects of FCN against PQ\u2010induced hepatic injury both in vivo and vitro. Mice in PQ group were injected with PQ, MIHA cells in PQ group were exposed to PQ for 24\u2009h, for the establishment of hepatic injury models. Hepatic injuries were assayed by serum alanine aminotransferase (ALT)\/aspartate aminotransferase (AST), liver histology, and myeloperoxidase (MPO) activity. Hepatic oxidative stress was assayed by malondialdehyde (MDA), superoxide dismutase (SOD), Heme oxygenase\u20101 (HO\u20101), quinone oxidoreductase\u20101 (NQO\u20101), kelch\u2010like ECH\u2010associated protein 1 (Keap\u20101), and nuclear factor erythroid\u20102\u2010related factor 2 (Nrf2). Hepatic inflammation was assayed by nucleotide\u2010binding domain and leucine\u2010rich repeat containing protein 3 (NLRP3), Caspase\u20101, interferon (IL)\u221218, IL\u20106, IL\u20104, IL\u201010, tumor necrosis factor (TNF)\u2010\u03b1, inducible isoform of Nitric Oxide Synthase (iNOS), and nuclear factor kappa\u2010B (NF\u2010\u03baB) p\u2010p65. In mice, pre\u2010\/post\u2010 treatment of FCN mitigated PQ\u2010induced weight loss and higher liver\/weight ratio, hepatic injury (increased serum ALT\/AST concentrations, and MPO activity), oxidative stress (increased MDA content and Keap\u20101 protein level, impaired SOD activity, mRNA expressions of Nrf2, SOD1\/2, HO\u20101 and NQO\u20101, protein expressions of Nrf2 and HO\u20101), hepatic inflammation (increased mRNA expressions of NLRP3, Caspase\u20101, IL\u20106, TNF\u2010\u03b1, and IL\u201018, serum IL\u20106 and TNF\u2010\u03b1 contents, protein expression of p\u2010p65), and hepatic mitochondrial dysfunction (increased mRNA level of iNOS). Meanwhile, in MIHA cells, FCN dose\u2010dependently mitigated PQ\u2010induced oxidative stress (increased protein expression of Keap\u20101, decreased mRNA expressions of Nrf2, SOD1\/2, HO\u20101 and NQO\u20101, Nrf2 and HO\u20101 protein expressions), and inflammation (increased p\u2010p65 protein expression, IL\u20106, TNF\u2010\u03b1, and IL\u201018 mRNA expressions and decreased IL\u201010 and IL\u20104 mRNA expressions). FCN prevents PQ\u2010induced hepatic injury by attenuating oxidative stress and inflammation via activating Nrf2\/Keap\u2010l signaling in vivo and in vitro.<\/jats:p>","DOI":"10.1002\/jbt.70574","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T16:17:43Z","timestamp":1761754663000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Fucoidan Prevents Paraquat\u2010Induced Hepatic Injury by Attenuating Oxidative and Inflammatory Stress: An In Vivo and In Vitro Approach"],"prefix":"10.1002","volume":"39","author":[{"given":"Ming","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Emergency Affiliated Hospital of Jiangnan University Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minmin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Unit Affiliated Hospital of Jiangnan University Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Emergency Affiliated Hospital of Jiangnan University Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Emergency Affiliated Hospital of Jiangnan University Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong Yan","family":"Qiao","sequence":"additional","affiliation":[{"name":"Department of Medical Imaging Affiliated Hospital of Jiangnan University Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.59556\/japi.73.0846"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.22441"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-29800-0"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101606"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules20045456"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408440701669959"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/MS9.0000000000001166"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ccr3.5020"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2018.04.151"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwm014"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/md14080145"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym15081912"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26071937"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.29219\/fnr.v65.5384"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods13101523"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/md14030042"},{"key":"e_1_2_11_18_1","unstructured":"Re: GRAS Notice No. GRN 000661 accessed July 4 2019 https:\/\/www.fda.gov\/downloads\/Food\/IngredientsPackagingLabeling\/GRAS\/NoticeInventory\/ucm549588.pdf."},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-020-01234-1"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.03.245"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2022.114411"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2022.154241"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2020.00201"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.80743"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjpp-2021-0488"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2022.102389"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.14348\/molcells.2023.0028"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.2103.03057"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1156995"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00350.2018"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2022.109130"},{"issue":"7","key":"e_1_2_11_32_1","first-page":"766","article-title":"Inducible Nitric Oxide Synthase in the Liver: Regulation and Function","volume":"63","author":"Taylor B. 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