{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T20:25:53Z","timestamp":1792095953554,"version":"4.4.0"},"reference-count":67,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T00:00:00Z","timestamp":1762732800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Bladder cancer (BC) is a prevalent malignancy worldwide. Cisplatin (Cis)\u2010based chemotherapy is the principal therapeutic method for many malignancies, including BC. However, side effects and resistance to Cis therapy pose significant challenges in the treatment of BC. Sitagliptin (Sita), an FDA\u2010approved hypoglycemic drug that functions as a DPP\u20104 inhibitor, has recently received attention in cancer therapy. This study aimed to explore the effects of combined treatment of Cis and Sita on the BC cell line HTB\u20109. In this study, the IC\n                    <jats:sub>50<\/jats:sub>\n                    values of Sita and Cis were quantified using an MTT assay. The cells were assigned into four groups: control, Cis, Sita, and Cis + Sita. HTB\u20109 cells were incubated with Sita and\/or Cis for 72\u2009h. Gene expression was assessed via real\u2010time PCR, while protein expression was evaluated by Western blot analysis. The IC\n                    <jats:sub>50<\/jats:sub>\n                    values for Cis and Sita were 3.01 and 543\u2009\u03bcg\/ml, respectively. Treatment of HTB\u20109 cells with Sita or Cis, alone or in combination, led to a significant decline in the mRNA and protein expression of proliferation\u2010dependent markers such as AKT, PI3K, and mTOR. In addition, a notable decrease in mRNA and protein expression of the antiapoptotic marker Bcl\u20102 was observed in all treated groups. In contrast, the protein expression of proapoptotic markers, including Bax, Caspase\u20108, Caspase\u20103, and Caspase\u20107 increased after treatment with Sita and\/or Cis. The findings indicated that the combination treatment with Sita and Cis had the most pronounced effect on suppressing cell proliferation and inducing apoptosis. Overall, it can be suggested that Sita may be a promising candidate for combination therapy with Cis in the treatment of BC.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70584","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T08:48:51Z","timestamp":1762764531000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Exploring the Effectiveness of Sitagliptin to Enhance Cisplatin Chemosensitivity in HTB\u20109 Bladder Cancer Cells"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6573-0835","authenticated-orcid":false,"given":"Shokooh","family":"Mohtadi","sequence":"first","affiliation":[{"name":"Cellular and Molecular Research Center, Medical Basic Sciences Research Institute Ahvaz Jundishapur University of Medical Sciences Ahvaz Khuzestan Iran"},{"name":"Toxicology Research Center, Medical Basic Sciences Research Institute Ahvaz Jundishapur University of Medical Sciences Ahvaz Khuzestan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahrzad","family":"Molavinia","sequence":"additional","affiliation":[{"name":"Toxicology Research Center, Medical Basic Sciences Research Institute Ahvaz Jundishapur University of Medical Sciences Ahvaz Khuzestan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeedeh","family":"Shariati","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Research Center, Medical Basic Sciences Research Institute Ahvaz Jundishapur University of Medical Sciences Ahvaz Khuzestan Iran"},{"name":"Toxicology Research Center, Medical Basic Sciences Research Institute Ahvaz Jundishapur University of Medical Sciences Ahvaz Khuzestan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masoud","family":"Mahdavinia","sequence":"additional","affiliation":[{"name":"Toxicology Research Center, Medical Basic Sciences Research Institute Ahvaz Jundishapur University of Medical Sciences Ahvaz Khuzestan Iran"},{"name":"Department of Toxicology, Faculty of Pharmacy Ahvaz Jundishapur University of Medical Sciences Ahvaz Khuzestan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5893-6379","authenticated-orcid":false,"given":"Dian","family":"Dayer","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Research Center, Medical Basic Sciences Research Institute Ahvaz Jundishapur University of Medical Sciences Ahvaz Khuzestan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farnoosh","family":"Farzam","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Biological Sciences Tarbiat Modares University Tehran Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,10]]},"reference":[{"issue":"1","key":"e_1_2_10_2_1","first-page":"15","article-title":"Epidemiology of Bladder Cancer","volume":"8","author":"Saginala K.","year":"2020","journal-title":"Medical Sciences (Basel, Switzerland)"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2024.07.027"},{"issue":"1","key":"e_1_2_10_4_1","first-page":"485","article-title":"Risk Factors for Bladder Cancer: Results of a Survey of Hospital Patients","volume":"9","author":"Shadab R.","year":"2023","journal-title":"Journal of Cancer & Allied Specialties"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2024.116797"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/btm2.10353"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2014.07.025"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-022-01517-9"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-13-2805"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.5402\/2012\/137289"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.2147\/JEP.S267383"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2023.102754"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/1534735404270335"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2024.106939"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41585-020-00404-6"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.euf.2022.02.007"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0dt00292e"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12645-023-00221-7"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23031532"},{"key":"e_1_2_10_20_1","doi-asserted-by":"crossref","unstructured":"M.Rizzo M. 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