{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T04:09:15Z","timestamp":1791259755444,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T00:00:00Z","timestamp":1762387200000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    1,2,4\u2010Triazines exhibit various pharmacological properties due to their strong biological activities, such as anticancer, anti\u2010inflammatory, antimicrobial, antiviral, and antioxidant, and also occupy an important place in the pharmaceutical field. The tested compounds were synthesized and characterized. 1,2,4\u2010Triazines were evaluated for their inhibitory and cytotoxicity effects on acetylcholinesterase (AChE), glutathione S\u2010transferase (GST), human liver cancer cell line (HepG2), and human colorectal adenocarcinoma cell line (HT\u201029). 1,2,4\u2010Triazines (\n                    <jats:bold>3a<\/jats:bold>\n                    \u2010\n                    <jats:bold>c<\/jats:bold>\n                    ,\n                    <jats:bold>5<\/jats:bold>\n                    ,\n                    <jats:bold>6<\/jats:bold>\n                    ,\n                    <jats:bold>10<\/jats:bold>\n                    ) were found to inhibit these enzymes with IC\n                    <jats:sub>50<\/jats:sub>\n                    values ranging from 1.41 to 4.28\u2009\u03bcM for AChE and from 1.58 to 4.13\u2009\u03bcM for GST. 1,2,4\u2010Triazine compounds were tested in range of 12.5\u2212100\u2009\u00b5M concentrations against HepG2 and HT\u201029 cell lines. Among the 1,2,4\u2010triazines tested,\n                    <jats:bold>10<\/jats:bold>\n                    was the most effective substance on HepG2 cells, especially at high doses, while\n                    <jats:bold>3b<\/jats:bold>\n                    was effective on HT\u201029. Furthermore, molecular docking results indicated that the 1,2,4\u2010triazines showed strong binding and stability at the active site of enzymes and selected proteins. 1,2,4\u2010Triazines may serve as promising precursors for the design of potent enzyme inhibitors and anticancer agents.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70591","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T16:36:48Z","timestamp":1762447008000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Design, Synthesis, and Characterization of Novel Substituted 1,2,4\u2010Triazines: Cytotoxic Activity on HepG2 and HT\u201029 Cell Lines, Enzyme Inhibition, and Molecular Docking Studies"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4838-1503","authenticated-orcid":false,"given":"Adnan","family":"Cetin","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Education Van Yuzuncu Yil University Van T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ercan","family":"Oguz","sequence":"additional","affiliation":[{"name":"Department of Medical Services and Techniques, Health Services Vocational School Igd\u0131r University Igd\u0131r T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Woli\u0144ska","sequence":"additional","affiliation":[{"name":"Institute of Chemical Sciences, University of Siedlce Siedlce Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Przemys\u0142aw","family":"Rozbicki","sequence":"additional","affiliation":[{"name":"Institute of Chemical Sciences, University of Siedlce Siedlce Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danuta","family":"Branowska","sequence":"additional","affiliation":[{"name":"Institute of Chemical Sciences, University of Siedlce Siedlce Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fikret","family":"T\u00fcrkan","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences, Faculty of Dentistry Igd\u0131r University Igd\u0131r T\u00fcrkiye"},{"name":"Department of Basic Medical Science, Medical of Faculty Nakhichevan State University Nakhichevan Azerbaijan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/07357907.2024.2361295"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3391"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1515\/hsz-2017-0131"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1949"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.2147\/VHRM.S33194"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jjco\/hyy053"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10728-009-0112-5"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/slct.202203365"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2024.116409"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2021.1969287"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmcr.2024.100154"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2017.04.064"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2024.139733"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00044-024-03251-x"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2019.e01422"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.7b00930"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-016-0279-9"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.30.152"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/P19870001069"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.70510"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(61)90145-9"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2021.103449"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/13813455.2019.1618341"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.10.3879"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2020.104524"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2021.130989"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11030-021-10344-x"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/slct.202502666"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms160921681"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.29576"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2025.143622"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21334"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2022.05.009"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi962316i"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1917635117"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M403319200"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/cbdd.12697"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.70470"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1107\/S2053229622007781"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/ardp.202400182"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2018.07.016"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-55604-6"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2024.116185"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11030-024-10828-6"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1134\/S1070363222110263"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.70415"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.23259"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202200043"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-024-01607-9"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2023.105757"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00044-022-02974-z"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2025.141708"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032-025-02745-x"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.70591","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbt.70591","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.70591","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:27:19Z","timestamp":1785436039000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.70591"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":54,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1002\/jbt.70591"],"URL":"https:\/\/doi.org\/10.1002\/jbt.70591","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"2025-08-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70591"}}