{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T13:22:41Z","timestamp":1789392161484,"version":"build-2803163510"},"reference-count":59,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Colistin is a polymyxin glycopeptide antibiotic produced by bacteria known as colistinus, a\n                    <jats:italic>Bacillus polymixa<\/jats:italic>\n                    variant. Despite its deleterious side effects especially on the kidneys, the use of colistin was reconsidered because of its effectiveness against the multi\u2010drug\u2010resistant gram\u2010negative bacilli. Biochanin A (BCA) is an isoflavone phytoestrogen compound with well\u2010known anti\u2010inflammatory, antioxidant and antiapoptotic activities. The current study aimed to investigate the nephroprotective activities of BCA against renal injury induced by colistin. BCA was administered in two dose levels (25 and 50\u2009mg\/kg, p.o.) and colistin was injected at a daily dose of 480,000 IU\/kg, IP BCA administration guarded against colistin\u2010induced kidney injury as it inhibited the increase in serum levels of creatinine, urea and cystatin C as well as histopathological alterations. In addition, BCA It exhibited antioxidant activities as it ameliorated the increase in renal content of malondialdehyde (MDA) and the reduction in the activities of, superoxide dismutase (SOD), and catalase (CAT). This was associated with modulation of Bax and Bcl\u20102 mRNA expression so as to antagonize colistin\u2010induced apoptosis. Besides, BCA showed significant anti\u2010inflammatory actions against colistin\u2010 induced increase in the immunoreactivity of interleukin\u20101 beta (IL\u20101\u03b2), cyclooxygenase\u20102 (COX\u20102), and tumor necrosis factor\u2010alpha (TNF\u2010\u03b1). Further, BCA enhanced immuno\u2010expression of nuclear factor erythroid 2\u2013related factor 2 (Nrf2), heme oxygenase\u20101 (HO\u20101), and NAD(P)H: quinone oxidoreductase (NQO1) in colistin\u2010challenged animals. Also, BCA did not have any effects on the antibacterial activity of colistin. In conclusion, BCA significantly attenuates renal injury induced by the antibiotic colistin as evidenced by the reduced serum markers of kidney injury in rats. This can be credited, at least partially, to its antioxidant, antiapoptotic, anti\u2010inflammatory activites as well as induction of Nrf2\/HO\u20101\/NQO1 axis.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70616","type":"journal-article","created":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T18:10:07Z","timestamp":1763403007000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Biochanin A Inhibits Colistin\u2010Induced Kidney Injury in Rats via Induction of Nrf2\/HO\u20101\/NQO1 Axis"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4755-0742","authenticated-orcid":false,"given":"Mohammed Z.","family":"Nasrullah","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty of Pharmacy King Abdulaziz University Jeddah Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1086\/429323\/2\/M_40-9-1333-TBL001.GIF"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0B013E328332E672"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1592\/PHCO.30.12.1279"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/2110-5820-1-14"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/CC3995\/TABLES\/2"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1086\/599225\/2\/M_48-12-1724-TBL001.GIF"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0104-42302009000600023"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00343-13\/ASSET\/B66EBB05-3A37-4719-AD86-55F04D2B33D6\/ASSETS\/GRAPHIC\/ZAC9991019580006.JPEG"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/S00228-015-1865-4\/TABLES\/2"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/MOLECULES19010713"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.SCITOTENV.2020.137907"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1208\/AAPSJ080351"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-0760(01)00075-9"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/MOLECULES21050548"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.ANTIVIRAL.2012.10.004"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.EJPHAR.2010.11.026"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1271\/BBB.120675"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0115115"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.INTIMP.2018.05.010"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.18585\/INABJ.V16I3.3077"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.ETAP.2015.04.020"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/1440-1681.70000"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/MOLECULES21010069"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/0886022X.2016.1229995"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.4132\/JPTM.2016.08.08"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00213-21"},{"key":"e_1_2_11_28_1","unstructured":"I.Kaczmarczyk\u2010Sedlak M.Zych W.Wojnar et al. 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