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This study aimed to determine the effect of indole derivative 1\n                    <jats:italic>H<\/jats:italic>\n                    \u2010indole\u20102\u2010hydroxy\u20103\u2010carboxylic acid (IHCA), isolated as a novel alkaloid from\n                    <jats:italic>Capparis ovata<\/jats:italic>\n                    , on selected tumor suppressor, apoptotic, and cell cycle regulatory genes, which are known to be important in cancer pathophysiology, on Caco\u20102 and LNCaP cells in comparison with Taxol. The molecular mechanism of IHCA's anticancer activity is essentially undefined. Different concentrations of IHCA increased the expression levels of apoptosis\u2010related genes, including BCL\u20102 and TNF\u2010\u03b1. In addition, the tumor suppressor genes PTEN, P53, and RB were increased in LNCaP and Caco\u20102 cells. KRAS, an oncogenic gene, was significantly downregulated by IHCA in LNCaP cells. Western blot results showed that the protein expression levels of P53 and PTEN in LNCaP cells were increased when treated with IHCA, whereas CDK4 and TNF\u2010\u03b1 were decreased. Finally, IHCA and doxorubicin significantly increased P53\u2010driven luciferase activity compared to the control. The results strongly suggest that the novel natural compound IHCA has an anticancer effect involving the regulation of the P53 gene and its networks in vitro. The molecular docking and MD simulation analyses reveal that IHCA exhibits superior binding potential to the MDM2 protein compared to Nutlin\u20103a. MD simulations further confirm that IHCA maintains a more stable and consistent interaction with MDM2, as indicated by lower RMSD values and reduced ligand fluctuation. These results highlight IHCA's potential as a more effective MDM2 inhibitor, suggesting its promise as a lead compound for anticancer drug development.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Clinical Trial Registration:<\/jats:bold>\n                    Not applicable.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70666","type":"journal-article","created":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T07:40:41Z","timestamp":1767253241000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Small Indole Derivative Isolated From Caper (\n                    <i>Capparis ovata<\/i>\n                    ) as an Inducer of P53\u2010Mediated Apoptosis in Prostate Cancer: Comprehensive In Vitro and In Silico Studies"],"prefix":"10.1002","volume":"40","author":[{"given":"\u00d6zden \u00d6zg\u00fcn","family":"Acar","sequence":"first","affiliation":[{"name":"Health Services Vocational School of Higher Education Pamukkale University Denizli T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3283-1824","authenticated-orcid":false,"given":"I\u015f\u0131l","family":"Gazio\u011flu","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmacy Bezmialem Vakif University Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hatice","family":"Oru\u00e7","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Sciences Pamukkale University Denizli T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elif","family":"Kale","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Sciences Kocaeli University Kocaeli T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8333-035X","authenticated-orcid":false,"given":"Halil","family":"\u015eenol","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy Bezmialem Vakif University Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7946-6545","authenticated-orcid":false,"given":"G\u00fcla\u00e7t\u0131","family":"Top\u00e7u","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy &amp; 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