{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:25:04Z","timestamp":1777868704877,"version":"3.51.4"},"reference-count":56,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:00:00Z","timestamp":1767052800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:00:00Z","timestamp":1767052800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Beryllium sulfate (BeSO\n                    <jats:sub>4<\/jats:sub>\n                    ) is one of the leading members of beryllium family that can exert toxic effects via disrupting key signaling pathways and redox profile. Coumestrol (CML) is a polyphenolic compound with exceptional biological properties. This pre\u2010clinical investigation was performed to explore the cardioprotective ability of CML against BeSO\n                    <jats:sub>4<\/jats:sub>\n                    induced sub\u2010chronic cardiotoxicity. Thirty\u2010six male Sprague Dawley rats were categorized into distinct groups as follows: control, BeSO\n                    <jats:sub>4<\/jats:sub>\n                    (12\u2009mg\u2009kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), BeSO\n                    <jats:sub>4<\/jats:sub>\n                    (12\u2009mg\u2009kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    )\u2009+\u2009CML (5\u2009mg\u2009kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), and CML (5\u2009mg\u2009kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) treated group. It was observed that BeSO\n                    <jats:sub>4<\/jats:sub>\n                    administration upregulated the expression of cyclooxygenase\u20102 (COX\u20102), interleukin\u20101 receptor\u2010associated kinase1 (IRAK1), toll\u2010like receptor4 (TLR4), myeloid differentiation primary response protein88 (MyD88), nuclear factor\u2010kappa B (NF\u2010\u03baB), tumor necrosis factor receptor\u2010associated factor6 (TRAF6), tumor necrosis factor\u2010alpha (TNF\u2010\u03b1), I\u03baB kinase (IKK), and interleukin\u20101 beta (IL\u20101\u03b2), while suppressing the expression of inhibitor of nuclear factor kappa B alpha (I\u03baB\u03b1). The redox profile was altered through a significant reduction in enzymatic activities of HO\u20101, GPx, CAT, GSR, GST, and SOD, while a sudden escalation in the levels of ROS and MDA after BeSO\n                    <jats:sub>4<\/jats:sub>\n                    intoxication. The levels of cardiac injury markers such as ProBNP, CK\u2010MB, CPK, C\u2010reactive protein, troponin\u2010T, LDH, troponin\u2010I and BNP were elevated following the administration of BeSO\n                    <jats:sub>4<\/jats:sub>\n                    . Besides, BeSO\n                    <jats:sub>4<\/jats:sub>\n                    exposure increased the levels of Caspase\u20109, Bax, and Caspase\u20103 while reducing the levels of Bcl\u20102. Cardiac histology showed severe disruptions in BeSO\n                    <jats:sub>4<\/jats:sub>\n                    treated rats. Nonetheless, CML therapy alleviated aforementioned disruptions via regulating redox balance, inflammatory and apoptotic profile of cardiac tissues. These findings suggest that CML could be employed in clinical research to validate these findings in humans.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70671","type":"journal-article","created":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T17:32:00Z","timestamp":1767115920000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cardioprotective Potential of Coumestrol Against Beryllium Sulfate Induced Cardiotoxicity via Modulating IRAK1\/TRAF6 and TLR4\/MyD88 Axis in Sprague Dawley Rats"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-2946-6254","authenticated-orcid":false,"given":"Hesham M.","family":"Hassan","sequence":"first","affiliation":[{"name":"Department of Pathology, College of Medicine King Khalid University Abha Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Usman","family":"Hayder","sequence":"additional","affiliation":[{"name":"Department of Zoology, Wildlife and Fisheries University of Agriculture Faisalabad Faisalabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arifa","family":"Mehreen","sequence":"additional","affiliation":[{"name":"Department of Zoology, Wildlife and Fisheries University of Agriculture Faisalabad Faisalabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuad M.","family":"Alzahrani","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratories Sciences, College of Applied Medical Sciences Taif University Taif Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6688-0106","authenticated-orcid":false,"given":"Khalid J.","family":"Alzahrani","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratories Sciences, College of Applied Medical Sciences Taif University Taif Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khalaf F.","family":"Alsharif","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratories Sciences, College of Applied Medical Sciences Taif University Taif Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,30]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-031-84441-6_5"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/13880209.2022.2074051"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/CEJ.0000000000000526"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-021-00755-y"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26157598"},{"key":"e_1_2_11_7_1","first-page":"253","article-title":"Carcinogenicity and Genotoxicity of Lead, Beryllium, and Other Metals","volume":"1","author":"Cohen M. 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