{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:25:14Z","timestamp":1777868714459,"version":"3.51.4"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T00:00:00Z","timestamp":1768435200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Cyclophosphamide (CYP) is a widely\u2010used chemotherapeutic drug known to induce ovarian damage and infertility in women. The present study evaluated the potential protective roles of zofenopril, a sulfhydryl\u2010containing angiotensin\u2010converting enzyme inhibitor, and thymoquinone (ThQ), alone and in combination, against CYP\u2010induced ovarian damage in rats. Thirty adult female rats were divided into five groups: a control group that received normal saline; a CYP\u2010Induced group given a single intraperitoneal (IP) dose of 200\u2009mg\/kg CYP on day 17 of the experiment; and three groups pre\u2010treated with zofenopril, ThQ, or Zofenopril+ThQ for 17 days before CYP\u2010administration, and continuing for 2 days after CYP exposure. Animals were euthanized, and blood and ovarian tissues were collected 48\u2009h after CYP administration. Serum follicle\u2010stimulating hormone (FSH), luteinizing hormone (LH), anti\u2010M\u00fcllerian hormone (AMH), and estradiol (E2) levels were measured, as well as the apoptotic biomarker caspase\u20103, total antioxidant capacity (TAOC), and tumor necrosis factor (TNF\u2011\u03b1). Ovarian histomorphometry was also assessed. CYP injection induced ovarian injury, characterized by reduced serum AMH and E2 levels, increased FSH and LH levels, oxidative stress, and increased levels of inflammatory and apoptotic markers. In addition, severe ovarian atrophy, regression and degradation of ovarian follicles, and atrophic follicles were observed. Zofenopril and ThQ administration significantly mitigated these CYP\u2010mediated effects, restoring hormone levels, reducing oxidative stress and inflammation, and improving ovarian histology compared with the CYP\u2010Induced group. Based on the present study, zofenopril and ThQ have protective effects against CYP\u2010induced ovarian injury; while co\u2010administration of zofenopril+ThQ was more effective at inhibiting CYP\u2010induced effects on TAOC, TNF\u2011\u03b1, and caspase\u20103 levels.<\/jats:p>","DOI":"10.1002\/jbt.70696","type":"journal-article","created":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T08:31:26Z","timestamp":1768465886000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protective Effects of Zofenopril, Thymoquinone and Their Co\u2010Administration Against Cyclophosphamide\u2010Induced Ovarian Toxicity in Rats"],"prefix":"10.1002","volume":"40","author":[{"given":"Karmand Salih","family":"Hamaamin","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, College of Pharmacy University of Sulaimani Sulaymaniyah 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