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In the present investigation, we evaluated the efficacy of PARP\u20101 inhibitors, 3\u2010aminobenzamide and Olaparib, in a rodent model of colitis\u2010associated colorectal cancer (CACC) induced by azoxymethane (AOM) and Dextran sulphate sodium (DSS). For model induction, male BALB\/c mice were provided DSS (3% w\/v) in three cycles within drinking water following an injection of AOM (10\u2009mg\/kg, intraperitoneally). A week after the DSS treatment, 3\u2010aminobenzamide (5, 10 and 20\u2009mg\/kg; i.p.) and Olaparib (10\u2009mg\/kg; orally) were given until sacrifice. PARP inhibitors reduced tumour progression by down\u2010regulating mRNA expression for inflammatory markers, PARP\u20101, NLRP3, ASC, Caspase\u20101, and TNF\u2010\u03b1. Immunoblotting showed modulation of beclin\u20101 expression, while transmission electron microscopy results revealed an increase in autophagosome numbers within tumour tissues. Immunofluorescence for Ki67 and immunoblotting of PCNA indicated elevated cell proliferation in the AOM\/DSS group, mitigated by the treatment with 3\u2010aminobenzamide (20\u2009mg\/kg) and Olaparib (10\u2009mg\/kg). Finally, DNA damage was also reduced by the intervention of 3\u2010aminobenzamide and Olaparib as indicated by decrease \u03b3H2AX expression. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and \u03b2\u2010catenin expression suggested decrease apoptosis within tumour tissue. Both 3\u2010aminobenzamide (20\u2009mg\/kg; i.p.) and Olaparib (10\u2009mg\/kg; orally) exhibited coloprotective effects by modulating PARP\u20101\u2010NLRP3 inflammasome and autophagy pathways in a model of colitis\u2010associated colorectal cancer.<\/jats:p>","DOI":"10.1002\/jbt.70698","type":"journal-article","created":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T08:37:01Z","timestamp":1768466221000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Pharmacological Targeting of PARP\u20101\u2010NLRP3 Inflammasome Pathway in Colitis\u2010Associated Colorectal Neoplasia: Effects of 3\u2010aminobenzamide and Olaparib"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2729-2854","authenticated-orcid":false,"given":"Shivani","family":"Singla","sequence":"first","affiliation":[{"name":"Facility for Risk Assessment and Intervention Studies, Department of Pharmacology and 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