{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T10:15:45Z","timestamp":1771236945383,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T00:00:00Z","timestamp":1769385600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T00:00:00Z","timestamp":1769385600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    tRNA\u2010derived small RNAs (tsRNAs) have emerged as important regulators in cancer biology, yet their role in breast cancer remains insufficiently understood. This study identifies tRF\u201030\u2010PW5SVP9N15WV as a tsRNA consistently upregulated in breast cancer, supported by analyses of TCGA\u2010BRCA and independent clinical cohorts. Elevated expression was an independent predictor of poor overall survival (HR\u2009=\u200920.83, 95% CI 2.76\u2013157.35;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001) and displayed superior prognostic power compared with TNM stage. A nomogram integrating tRF\u201030\u2010PW5SVP9N15WV achieved a C\u2010index of 0.78, with time\u2010dependent ROC AUCs of 0.882, 0.733, 0.704, and 0.662 at 1, 3, 5, and 10 years, respectively, outperforming clinical parameters alone. Functional assays demonstrated that tRF\u201030\u2010PW5SVP9N15WV promotes proliferation (CCK\u20108, colony formation) and invasion (Transwell migration) in vitro, while xenograft and tail\u2010vein injection models confirmed its ability to accelerate tumor growth and lung metastasis (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01) in vivo. Subcellular fractionation revealed predominant cytoplasmic localization (67.1%), consistent with posttranscriptional regulatory activity. Clinically, tRF\u201030\u2010PW5SVP9N15WV also exhibited diagnostic potential in differentiating malignant from benign tissues (AUC\u2009=\u20090.805). Together, these findings establish tRF\u201030\u2010PW5SVP9N15WV as a clinically relevant tsRNA with diagnostic, prognostic, and therapeutic relevance in breast cancer.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70709","type":"journal-article","created":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T13:23:51Z","timestamp":1769433831000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transfer RNA\u2010Derived Small RNAs: Novel Predictive Biomarkers for Diagnosis and Prognosis of Breast Cancer"],"prefix":"10.1002","volume":"40","author":[{"given":"Lihan","family":"Bie","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine The Seventh People's Hospital Affiliated to Shanghai University of Traditional Chinese Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minhua","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of 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