{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T15:51:38Z","timestamp":1790610698155,"version":"4.1.0"},"reference-count":65,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Neuropathic pain is a complex chronic condition resulting from injury to the nerve. Paclitaxel (PTX) is a commonly used chemotherapeutic drug, but it is usually associated with peripheral neuropathy. Costunolide (COS), a bioactive sesquiterpene lactone, exhibits potent neuroprotective, anti\u2010inflammatory, antioxidant, and anti\u2010apoptotic properties. The current study aimed to investigate the efficacy of COS in attenuating PTX\u2010induced neuropathic pain using advanced spectroscopic, histological and biochemical analyses. Findings from the study revealed that administration with COS significantly improved the pain parameters, including mechanical allodynia, thermal hyperalgesia, and cold allodynia by increasing the pain thresholds. Muscle strength and motor coordination also improved significantly following administration of COS. Results from the Raman spectral analysis revealed distinct PTX\u2010induced biochemical disruptions, especially in the lipids and proteins, which were subsequently normalized by COS. FTIR spectra demonstrated that COS mitigated PTX\u2010induced damage to the myelin sheath, hence preserving the biochemical integrity. The tissue oxygenation (StO\n                    <jats:sub>2<\/jats:sub>\n                    ) profile was also improved. COS preserved neuronal architecture, reduced PTX\u2010induced apoptosis, and maintained spinal cord and sciatic nerve structure. It also increased the level of antioxidants, that is, GSH, GST, and catalase, while reducing the level of oxidative stress markers (MPO, EPO, LPO, NO). Together, these findings establish COS as a promising candidate for the treatment of peripheral neuropathy. Its multimodal protective mechanisms\u2014captured at both molecular and cellular levels\u2010 highlight its potential to counteract PTX\u2010induced neurotoxicity.\n                  <\/jats:p>","DOI":"10.1002\/jbt.70718","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T09:45:12Z","timestamp":1769766312000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Experimental Validation and Multimodal Spectroscopic Profiling of the Neuroprotective Potential of Costunolide in Chemotherapy\u2010Induced Neuropathic Pain"],"prefix":"10.1002","volume":"40","author":[{"given":"Omeyya","family":"Tanveer","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Pharmacological Sciences Research Lab, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"},{"name":"Department of Pharmacy, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeju","family":"Lee","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Graduate School of Pharmaceutical Sciences Ewha Womans University Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sana","family":"Zafar","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Pharmacological Sciences Research Lab, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"},{"name":"Department of Pharmacy, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Ibrar","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Pharmacological Sciences Research Lab, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"},{"name":"Department of Pharmacy, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"},{"name":"Department of Pharmacology, Faculty of Pharmacy Capital University of Science &amp; Technology Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amber","family":"Sani","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Pharmacological Sciences Research Lab, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"},{"name":"Department of Pharmacy, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahid","family":"Raza","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Pharmacological Sciences Research Lab, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"},{"name":"Department of Pharmacy, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raja Shahamad","family":"Tanveer","sequence":"additional","affiliation":[{"name":"Govt. Khawaja Muhammad Safdar Medical College Sialkot Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rimsha","family":"Noor","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kifayat Ullah","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9537-4897","authenticated-orcid":false,"given":"Fakhar ud","family":"Din","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"},{"name":"Department of Pharmacy, Nanomedicine Research Group, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hussain","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun Kyoung","family":"Seo","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Graduate School of Pharmaceutical Sciences Ewha Womans University Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salman","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Pharmacological Sciences Research Lab, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"},{"name":"Department of Pharmacy, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,30]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2011.06.017"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/187152409787601932"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.115413"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2023.113742"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-091740-5.50014-4"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2004.12.009"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep17165"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201900135"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5CS00581G"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-022-03565-5"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.22755"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1364\/OSAC.410807"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-34054-6"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20122926"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8FO02249F"},{"key":"e_1_2_13_17_1","first-page":"1079","article-title":"Costunolide Inhibits Proinflammatory Cytokines and iNOS in Activated Murine BV2 Microglia","volume":"3","author":"Rayan N. 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